پديدآورندگان :
Bagheri Nafiseh bagheri.nafiseh25@gmail.com Research Laboratory of Advanced Water and Wastewater Treatment Processes, Department of Applied Chemistry, Faculty of Chemistry, University of Tabriz, 51666-16471 Tabriz, Iran / Electroanalytical Chemistry Laboratory, Department of Chemistry, Faculty of Science, Azarbaijan Shahid Madani University, 53714-161 Tabriz, Iran; , Khataee Alireza Research Laboratory of Advanced Water and Wastewater Treatment Processes, Department of Applied Chemistry, Faculty of Chemistry, University of Tabriz, 51666-16471 Tabriz, Iran , Hassanzadeh Javad Research Laboratory of Advanced Water and Wastewater Treatment Processes, Department of Applied Chemistry, Faculty of Chemistry, University of Tabriz, 51666-16471 Tabriz, Iran , Habibi Biuck Electroanalytical Chemistry Laboratory, Department of Chemistry, Faculty of Science, Azarbaijan Shahid Madani University, 53714-161 Tabriz, Iran
كليدواژه :
Metal organic frameworks , , nanocomposite , colorimetric technique , cholesterol.
چكيده فارسي :
Development of a nano-mimics with intrinsic peroxidase-like catalytic activity have been extensively reported [1]. The metal organic frameworks (MOFs) with a porous crystalline structures are inorganic polymers with stable porous structure and a great surface area, providing a superior catalytic activity [2, 3]. Here, Ag nanoparticle/flake-like Zn-based MOF nanocomposite (AgNPs@ZnMOF) with great peroxidase-like activity was applied as an efficient peroxidase-like material for sensitive determination of cholesterol. AgNPs@ZnMOF was simply synthesized by creating AgNPs inside the nano-pores of flake-like ZnMOF. The high surface area of MOF remarkably improved the catalytic activity of AgNPs which was assessed by colorimetric technique. The combination of exceptional peroxidase mimetic activity of MOF-based nanocomposites and simple colorimetric detection methods creates a novel attractive investigation field [4]. On the other hand, it s well-known that enzymatic oxidation of cholesterol by cholesterol oxidase lead to the production of H2O2. We applied the produced H2O2 for subsequent oxidization of 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of AgNPs@ZnMOF as a catalyst. This reaction led to the production of a blue colored product which was recognized by the colorimetric technique. The effect of some important factors (for example pH, buffer, TMB concentration etc.) were optimized to obtain maximum sensitivity. A linear relationship was obtained between the absorption intensity of the produced blue solution and the cholesterol concentration in the range of 0.5-120 µM, with a detection limit of 0.3 µM.
