High mimetic HKUST-1 MOF for the fluorometric determination of glucose

Developing a novel peroxidase nano-mimetic is a challenging research topic in bio-sensing field [1], and metal organic frameworks (MOFs) with a great surface area are good candidate for this purpose. They are porous inorganic polymers with crystalline structures which have stable porous structure providing a superior catalytic activity [2, 3]. Herein, HKUST-1 flake-like metal-organic framework (MOF) was introduced as a nano-porous material with improved peroxidase mimetic behavior and used for selective determination of glucose. The catalytic activity of this nano-porous material in chemical reduction of H2O2 was studied using fluorometric technique. First, it was based on the enzymatic oxidation of glucose using glucose oxidase and then, based on the simple measurement of produced H2O2 using terephthalic acid fluorescence system as sensitive detection method. In optimum condition, a linear association was found between the generated fluorescence intensity and glucose concentration in the range of 0.02-600 µM, and the limit of detection (3S/m) was 40 nM. The developed method had superior properties and was successfully applied for the detection of glucose level in human blood.