Determination and Quantitation of Methadone in Syrup by Ion Mobility Spectrometry

Methadone hydrochloride is used for the treatment of severe pain and for the treatment of dependence on opioid drugs. pharmacological dosage regimens is a prerequisite to the success of most treatments [1]. Methadone syrup contains the various ingredients. Concentration of Methadone hydrochloride in syrup should be 5mg/mL. Furthermore, variable dose of it hasn’t effect on patients treatment. Therefore, methadone dosage should be individually determined and quantitated [2]. In this work, First, the extraction steps optimized and then methadone concentration determined with gas chromatography- mass spectroscopy (GC-MS) and Ion mobility spectrometry (IMS) methods and then, the resultes were compared together. In this study, IMS was developed to quantitate methadone hydrochloride in syrup. The aim of this work has been the development of ion mobility spectrometry (IMS) technique which designed and constructed in Institute of Materials and Energy, preceded by a extraction step optimized for the analysis of methadone hydrochloride. Several samples of the syrup were extracted and analyzed with these two methods. Test results of IMS and GC-MS were compared with statistical and chemometrics methods. Results revealed that the performance of IMS and GC-MS in identification and quantification of methadone in syrup are almost the same.