پديدآورندگان :
Pashazadeh Ali Electroanalytical Chemistry Research Lab., Faculty of Basic Sciences, Azarbaijan Shahid Madani University, Tabriz, Iran , Pashazadeh Sara Electroanalytical Chemistry Research Lab., Faculty of Basic Sciences, Azarbaijan Shahid Madani University, Tabriz, Iran , Bahadori Younes Electroanalytical Chemistry Research Lab., Faculty of Basic Sciences, Azarbaijan Shahid Madani University, Tabriz, Iran , Habibi Biuck B.Habibi@azaruniv.ac.ir Electroanalytical Chemistry Research Lab., Faculty of Basic Sciences, Azarbaijan Shahid Madani University, Tabriz, Iran;
كليدواژه :
Copper nanoparticles , glassy carbon electrode , electrooxidation , isoniazid
چكيده فارسي :
In the present investigation a new electrochemical sensor based on copper nanoparticles for detection of isoniazid (INZ) has been developed. Copper nanoparticles/glycine/graphene oxide nanosheets (GONs) modified glassy carbon electrode (CuNPs/glycine/GONs/GCE) was prepared by using dripping well-dispersed graphene oxide nanosheets, electropolymerized poly glycine (p-Gly) followed by electroplating of metallic copper nanoparticle film. The CuNPs/glycine/GONs modified glassy carbon electrode has excellent electrocatalytic ability for the electrooxidation of isoniazid. Scanning electron microscopy and energy dispersive X-ray spectrometry were used for the characterization of the prepared film. The process of oxidation and its kinetics were established by using cyclic voltammetry, chronoamperometry and differential pulse voltammetry (DPV) techniques. The results indicate that CuNPs/glycine/GONs can facilitate the electrochemical oxidation of isoniazid with great decrease of overpotential in pH 7.0 phosphate buffer solution. The proposed biosensor provides excellent performance towards the determination of isoniazid with acceptable linear response ranges from 0.2 to 60 μM with the limit of detection (LOD) of 6.07 μM. Studies show the facts that oxidation of INZ occurs through a four proton transferring mechanism and endures a limitation, considered to be depended on generation of N2 as the final oxidation product [1, 2]. The diffusion coefficient of 3.65×10−5 cm2 s-1 for isoniazid was also estimated using chronoamperometry study. The CuNPs/glycine/GONs modified electrode displayed good storage stability, excellent reproducibility toward the sensing of INZ. The utility of this modified electrode was demonstrated for the determination of INZ in solid pharmaceutical formulations. The results of the analysis suggest that the proposed method has promise for the routine determination of isoniazid in the products examined.
