Salting-out liquid liquid microextraction of determination of mycophenolic acid using terbium-sensitized luminescence method

Mycophenolic Acid (MPA) is an immunosuppressant drug used to prevent organ transplant rejection and for the treatment of autoimmune diseases.[1] Developing of analysis methods for therapeutic drug monitoring (TDM) is an important issue for measuring of drugs in biological samples.[2] Salting-out assisted liquid–liquid extraction is one of the simple methods in bioanalysis which applies the salting-out effect to separate water-miscible organic solvent such as acetonitrile from plasma.[3] In addtion, terbium-sensitized luminescence methods are one of the simple and sensitive method for the determination of drugs in biological samples.[4] This study aimed to develop an analysis method for determination of MPA in biological samples using salting-out liquid liquid microextraction from plasma samples based on the formation of a fluorescent complex with terbium ions.Under the optimum conditions for the developed analysis method, the method provided a linear range between 0.5 to 5 mg/L with a correlation coefficient (R2) of 0.998 with a limit of detection (LOD) and limit of quantification (LOQ) of 0.1 mg/L and 0.33 mg/L, respectively.