آﻧﺎﻟﯿﺰ ﺑﯿﻮاﻧﻔﻮرﻣﺎﺗﯿﮑﯽ ﺧﺎﻧﻮاده ژﻧﯽ ﺳﯿﻨﺎﻣﻮﯾﻞ ﮐﻮآﻧﺰﯾﻢ آ ردوﮐﺘﺎز CCR در ﺳﻮﯾﺎ

Bioinformatics analysis of cinnamoyl-CoA reductase (CCR) gene family in soybean

ﺧﺎﻧﻮاده ژﻧﯽ CCR از 18 ژن ﺗﺸﮑﯿﻞ ﺷﺪه اﺳﺖ ﮐﻪ ﺑﺮ روي ﮐﺮوﻣﻮزومﻫﺎي ﻣﺨﺘﻠﻒ ﻗﺮار ﮔﺮﻓﺘﻪاﻧﺪ. اﯾﻦ ژنﻫﺎ ﭘﺮوﺗﺌﯿﻦﻫﺎﯾﯽ را ﮐﺪ ﻣﯽﮐﻨﻨﺪ ﮐﻪ در ﻣﺴﯿﺮ ﺑﯿﻮﺳﻨﺘﺰ ﻟﯿﮕﻨﯿﻦ ﻧﻘﺶ دارﻧﺪ. ﺟﻬﺖ آﻧﺎﻟﯿﺰ ژنﻫﺎي اﯾﻦ ﺧﺎﻧﻮاده، ﻣﻮﻗﻌﯿﺖ ژنﻫﺎ روي ﮐﺮوﻣﻮزومﻫﺎ و وﯾﮋﮔﯽ-ﻫﺎي آنﻫﺎ ﺷﻨﺎﺳﺎﯾﯽ ﺷ.ﺪ ﺳﭙﺲ، ﻋﻤﻠﮑﺮد و رﻣﺰﮔﺬاري ﭘﺮوﺗﺌﯿﻦﻫﺎي آنﻫﺎ و ﻫﻤﭽﻨﯿﻦ ﻣﺤﻞ ﻓﻌﺎﻟﯿﺖ و ﺟﺎﯾﮕﯿﺮي ﭘﺮوﺗﺌﯿﻦﻫﺎ و ﻗﺴﻤﺖﻫﺎي ﻏﺸﺎﯾﯽ، درون و ﺧﺎرج ﺳﻠﻮﻟﯽ آنﻫﺎ و وﺟﻮد ﺳﯿﮕﻨﺎل ﺗﺮﺷﺤﯽ ﻣﺸﺨﺺ ﺷﺪ. ﻋﻼوه ﺑﺮ آن ﭘﺎﯾﺪاري و ﻋﺪم ﭘﺎﯾﺪاري، ﺷﺎﺧﺺ آﻟﯿﻔﺎﺗﯿﮏ و ﻧﻘﻄﻪ اﯾﺰواﻟﮑﺘﺮﯾﮏ ﭘﺮوﺗﺌﯿﻦﻫﺎي ﮐﺪ ﺷﺪه ﺗﻮﺳﻂ اﯾﻦ ژنﻫﺎ ﺗﻌﯿﯿﻦ ﺷ.ﺪ ﻧﺘﺎﯾﺞ ﻧﺸﺎن داد ﮐﻪ ﻣﺤﻞ ﺟﺎﯾﮕﯿﺮي ﺑﺮﺧﯽ از اﯾﻦ ﭘﺮوﺗﺌﯿﻦﻫﺎ در ﮐﻠﺮوﭘﻼﺳﺖ، ﺷﺒﮑﻪ آﻧﺪوﭘﻼﺳﻤﯽ، ﻫﺴﺘﻪ و ﺑﺮﺧﯽ دﯾﮕﺮ در ﻣﺴﯿﺮﻫﺎي ﺗﺮﺷﺤﯽ ﻣﯽﺑﺎﺷﺪ. اﯾﻦ ﭘﺮوﺗﺌﯿﻦﻫﺎ ﺧﺎرج ﺳﻠﻮﻟﯽ ﺑﻮده و ﻏﺸﺎﯾﯽ ﻧﯿﺴﺘﻨ.ﺪ ﻫﻤﭽﻨﯿﻦ، ﺗﻤﺎﻣﯽ اﯾﻦ ﭘﺮوﺗﺌﯿﻦﻫﺎ داراي ﺳﯿﮕﻨﺎل ﺗﺮﺷﺤﯽ ﻣﯽﺑﺎﺷﻨ.ﺪ ﺑﯿﺸﺘﺮ ﭘﺮوﺗﺌﯿﻦﻫﺎي اﯾﻦ ﺧﺎﻧﻮاده ژﻧﯽ ﭘﺎﯾﺪار ﻫﺴﺘﻨﺪ.

The CCR gene family consists of 18 genes located on different chromosomes. These genes encode proteins that are involved in the lignin biosynthesis pathway. To analyze the genes in this family, the position of the genes on the chromosomes and their characteristics were identified.Then, the function and encoding of their proteins, as well as the location and activity of the membrane, intracellular and extracellular proteins and their secretory signal were determined. In addition, stability and instability, aliphatic index and isoelectric point of the proteins encoded by these genes were determined.The results showed that some of these proteins are located in chloroplast, endoplasmic reticulum, nucleus and others in secretory pathways.These proteins are extracellular and not membrane. Also, all of these proteins have secretory signals.Most proteins in this gene family are stable.