اﺛﺮ ﺑﺎﮐﺘﺮي ﻫﺎي ﻣﺤﺮك رﺷﺪ ﺑﺮ ﻣﻮﻟﻔﻪ ﻫﺎي ﺟﻮاﻧﻪ زﻧﯽ، رﺷﺪ ﮔﯿﺎﻫﭽﻪ و ﺷﺎﺧﺺﻫﺎي ﺑﯿﻮﺷﯿﻤﯿﺎﯾﯽ ﺟﻮ ﺗﺤﺖ ﺷﺮاﯾﻂ ﺗﻨﺶ ﺧﺸﮑﯽ

Effects of plant growth promoting rhizobacteria on germination traits, seedling growth and biochemical traits of barely under drought stress conditions

به منظور ﺑﺮرﺳﯽ ﺑﺎﮐﺘﺮي ﻫﺎي ﻣﺤﺮك رﺷﺪ ﺑﺮ ﻣﻮﻟﻔﻪ ﻫﺎي ﺟﻮاﻧﻪ زﻧﯽ، ﺷﺎﺧﺺ ﻫﺎي رﺷﺪ و ﺗﻐﯿﯿﺮات ﺑﯿﻮﺷﯿﻤﯿﺎﯾﯽ ﺟﻮ ﺗﺤﺖ ﺷﺮاﯾﻂ ﺗﻨﺶ آﺑﯽ، آزﻣﺎﯾﺸﯽ ﺑﻪ ﺻﻮرت ﻓﺎﮐﺘﻮرﯾﻞ در ﻗﺎﻟﺐ ﻃﺮح ﺑﻠﻮكﻫﺎي ﮐﺎﻣﻞ ﺗﺼﺎدﻓﯽ در 3 ﺗﮑﺮار در ﺳﺎل زراﻋﯽ 1396-97 در ﯾﺰد اﻧﺠﺎم ﺷﺪ. ﻓﺎﮐﺘﻮر اول ﺷﺎﻣﻞ 3 ﭘﯿﺶ ﺗﯿﻤﺎر ﻣﺨﺘﻠﻒ ﺑﺎﮐﺘﺮﯾﺎﯾﯽ )آزﺳﭙﺮﯾﻠﯿﻮم، ازﺗﻮﺑﺎﮐﺘﺮ و ﺷﺎﻫﺪ( و ﻓﺎﮐﺘﻮر دوم ﺷﺎﻣﻞ ﺳﻪ ﺳﻄﺢ ﺗﻨﺶ آﺑﯽ )ﺻﻔﺮ، 6- و 12- ﺑﺎر( ﺑﻮد. ﻧﺘﺎﯾﺞ ﻧﺸﺎن داد ﮐﻪ اﺛﺮ ﻣﺘﻘﺎﺑﻞ ﺑﺎﮐﺘﺮي و ﺗﻨﺶ ﺧﺸﮑﯽ ﺑﺮ درﺻﺪ ﺟﻮاﻧﻪ زﻧﯽ، ﺷﺎﺧﺺ ﺟﻮاﻧﻪ زﻧﯽ، ﻣﺘﻮﺳﻂ ﻣﺪت زﻣﺎن ﺟﻮاﻧﻪ زﻧﯽ، وزن ﺧﺸﮏ ﮔﯿﺎﻫﭽﻪ، ﺑﻨﯿﻪ ﺑﺬر، ﮐﺎﺗﺎﻻز، آﺳﮑﻮرﺑﺎت ﭘﺮاﮐﺴﯿﺪاز و ﭘﺮوﻟﯿﻦ ﻣﻌﻨﯽ دار ﺑﻮد. ﺑﺎﻻﺗﺮﯾﻦ درﺻﺪ ﺟﻮاﻧﻪ زﻧﯽ، ﺷﺎﺧﺺ ﺟﻮاﻧﻪ زﻧﯽ، وزن ﺧﺸﮏ ﮔﯿﺎﻫﭽﻪ، ﺑﻨﯿﻪ ﺑﺬر و ﮐﻤﺘﺮﯾﻦ ﻣﺘﻮﺳﻂ ﻣﺪت زﻣﺎن ﺟﻮاﻧﻪ زﻧﯽ ﻣﺮﺑﻮط ﺑﻪ ﺗﯿﻤﺎر ﺗﻠﻘﯿﺢ ﺑﺬر ﺑﺎ آزوﺳﭙﺮﯾﻠﯿﻮم و ﺷﺮاﯾﻂ ﺑﺪون ﺗﻨﺶ ﺑﻮد. اﮔﺮﭼﻪ اﻋﻤﺎل ﺗﻨﺶ ﺑﺎﻋﺚ اﻓﺰاﯾﺶ ﻓﻌﺎﻟﯿﺖ ﮐﺎﺗﺎﻻز، آﺳﮑﻮرﺑﺎت ﭘﺮاﮐﺴﯿﺪاز و ﻣﯿﺰان ﭘﺮوﻟﯿﻦ ﺷﺪ، اﺳﺘﻔﺎده از ﺑﺎﮐﺘﺮي ﻫﺎي ﻣﺤﺮك رﺷﺪ ﺳﺒﺐ ﮐﺎﻫﺶ آن ﻫﺎ ﺷﺪ.

The objective of this research was to evaluate the effects of seed-bacterial priming (inoculation) on germination traits, seedling growth and biochemical changes of barely under drought stress conditions. The experiment was carried out in factorial arrangement based on randomized complete block design (RCBD) with 3 replications. The first factor was priming using bacteria (Azetobacter, Azospirillum, control) and second factor was three levels of drought stress (0, -6 and -12 bar). Results showed that interactive effects of stress and priming was significant on all traits. The highest germination percentage, germination index, seedling dry weight, seed vigor and the minimum mean time for germination were attained from priming by Azospirillum and control conditions. Although, drought stress increased enzyme activities and proline content, using Azetobacter, Azospirillum could decrease the activity of enzymes and proline content.