ارزﯾﺎﺑﯽ اﺛﺮ ﻫﯿﻮﻣﯿﮏاﺳﯿﺪ ﺑﺮ ﻋﻤﻠﮑﺮدﻓﯿﺰﯾﻮﻟﻮژﯾﮏ دو ﮔﻮﻧﻪ ﻧﻌﻨﺎع ﺳﺒﺰ .Mentha spicataL و ﻧﻌﻨﺎع ﻓﻠﻔﻠﯽ .Mentha piperita L ﺗﺤﺖ ﺗﻨﺶ ﺧﺸﮑﯽ

Evaluation of the effect of humic acid on the physiologicalyeild of two species green mint (Mentha spicta L.) and peppermint (Mentha piperitha L.) under drought stress

ﺑﻪ ﻣﻨﻈﻮر ﺑﺮرﺳﯽ اﺛﺮ اﺳﯿﺪ ﻫﯿﻮﻣﯿﮏ ﺑﺮ ﺻﻔﺎت ﻓﯿﺰﯾﻮﻟﻮژﯾﮏ دو ﮔﻮﻧﻪ ﻧﻌﻨﺎع )ﺳﺒﺰ و ﻓﻠﻔﻠﯽ( ﺗﺤﺖ ﺷﺮاﯾﻂ ﺗﻨﺶ ﺧﺸﮑﯽ، آزﻣﺎﯾﺸﯽ ﺑﻪ ﺻﻮرت ﻓﺎﮐﺘﻮرﯾﻞ در ﻗﺎﻟﺐ ﻃﺮح ﮐﺎﻣﻼً ﺗﺼﺎدﻓﯽ در ﺳﻪ ﺗﮑﺮار در ﮔﻠﺨﺎﻧﻪ ﺗﺤﻘﯿﻘﺎﺗﯽ داﻧﺸﮑﺪه ﮐﺸﺎورزي داﻧﺸﮕﺎه ﺷﺎﻫﺪ ﺗﻬﺮان در ﺳﺎل 1397 اﻧﺠﺎم ﺷﺪ. ﻓﺎﮐﺘﻮرﻫﺎي آزﻣﺎﯾﺶ ﺷﺎﻣﻞ ﭼﻬﺎر ﺳﻄﺢ ﺗﻨﺶ ﺧﺸﮑﯽ )ﺑﺮاﺳﺎس آﺑﯿﺎري در 100، 75، 50 و 25 درﺻﺪ ﻇﺮﻓﯿﺖ زراﻋﯽ(، اﺳﯿﺪ ﻫﯿﻮﻣﯿﮏ در دو ﺳﻄﺢ )ﻋﺪم ﮐﺎرﺑﺮد و ﮐﺎرﺑﺮد 100 ﻣﯿﻠﯽﮔﺮم در ﻟﯿﺘﺮ( و دو ﮔﻮﻧﻪ ﻧﻌﻨﺎع ﺳﺒﺰ و ﻓﻠﻔﻠﯽ ﺑﻮدﻧﺪ. ﺻﻔﺎت ﻣﻮرد ﺑﺮرﺳﯽ در اﯾﻦ ﻣﯿﺰان ﮐﻠﺮوﻓﯿﻞ ،b a و ﮐﻞ و ﻣﺤﺘﻮي ﮐﺎرﺗﻨﻮﺋﯿﺪ ﺑﻮدﻧ.ﺪ آﺑﯿﺎري در 25 درﺻﺪ ﻇﺮﻓﯿﺖ زراﻋﯽ ﺑﺎﻋﺚ ﮐﺎﻫﺶ ﮐﻠﺮوﻓﯿﻞ a )33/6 درﺻﺪ(، ﮐﻠﺮوﻓﯿﻞ ﮐﻞ )26/1 درﺻﺪ( و ﮐﺎرﺗﻨﻮﺋﯿﺪ )58/6 درﺻﺪ( ﮔﺮدﯾﺪ. ﺑﯿﺸﺘﺮﯾﻦ ﻣﺤﺘﻮي ﮐﻠﺮوﻓﯿﻞ ﮐﻞ در ﮔﻮﻧﻪ ﻧﻌﻨﺎع ﻓﻠﻔﻠﯽ ﺗﺤﺖ ﺷﺮاﯾﻂ ﮐﺎرﺑﺮد اﺳﯿﺪ ﻫﯿﻮﻣﯿﮏ و ﺗﻨﺶ ﺧﺸﮑﯽ 75 درﺻﺪ ﻇﺮﻓﯿﺖ زراﻋﯽ ﺑﺎ ﻣﯿﺎﻧﮕﯿﻦ 54/0 ﻣﯿﮑﺮوﮔﺮم ﺑﺮ ﮔﺮم وزن ﺗﺮ ﺑ.ﻮد ﮐﻤﺘﺮﯾﻦ ﻣﯿﺎﻧﮕﯿﻦ اﯾﻦ ﺻﻔﺖ در ﮔﻮﻧﻪ ﻧﻌﻨﺎع ﺳﺒﺰ ﺗﺤﺖ ﺷﺮاﯾﻂ ﺗﻨﺶ 25 درﺻﺪ ﻇﺮﻓﯿﺖ زراﻋﯽ و ﻋﺪم ﮐﺎرﺑﺮد اﺳﯿﺪ ﻫﯿﻮﻣﯿﮏ ﺑﺎ ﻣﯿﺎﻧﮕﯿﻦ 21/8 ﻣﯿﮑﺮوﮔﺮم ﺑﺮ ﮔﺮم وزن ﺗﺮ ﻣﺸﺎﻫﺪه ﺷﺪ.

In order to investigate the effect of humic acid on physiological traits of two species of mint under drought stress conditions, a factorial experiment was conducted in a completely randomized design with three replications in a research greenhouse of Faculty of Agriculture, Shahed University of Tehran, in 2017. Experimental factors included four levels of drought stress (based on irrigation in 100, 75, 50 and 25% filed capacity), humic acid in two levels (no application and application of 100 mg/L), and two types of mint Mentha spicata L. and Mentha piperita L. The studied traits were chlorophyll a, b and total, and carotenoid contents. The results showed that drought stress had a significant effect on most traits. Irrigation in 25% filed capacity was caused reduceing chlorophyll a (33.6%), total chlorophyll (26.1%), and carotenoid (58.6%). The maximum content of total chlorophyll was achieved in peppermint under application of humic acid and drought stress of 75% field capacity with of 54.0 μg/g FW. The lowest mean of this trait was observed in green mint under 25% filed capacity and no application of humic acid with 21.8 μg/g FW