ﺑﺮرﺳﯽ ﺗﻨﻮع ژﻧﺘﯿﮑﯽ ﻻﯾﻦﻫﺎي دﺑﻠﺪﻫﺎﭘﻠﻮﺋﯿﺪ ﮐﺎﻣﻠﯿﻨﺎ ﺑﺮاي اﺳﯿﺪﻫﺎي ﭼﺮب

Genetic variation for fatty acids in doubled-haploid lines of camelina (Camelina sativa L.)

در اﯾﻦ ﺗﺤﻘﯿﻖ، ﺑﻪ ﺑﺮرﺳﯽ ﭘﺮوﻓﺎﯾﻞ، ﺟﺪاﺳﺎزي و ﺷﻨﺎﺳﺎﯾﯽ اﻧﻮاع اﺳﯿﺪ ﻫﺎي ﺑﺎارزش ﭼﺮب و ﭘﯿﺸﺮﻓﺖ و ﺑﻬﺮه ژﻧﺘﯿﮑﯽ آﻧﻬﺎ در 35 ﻻﯾﻦ داﺑﻠﺪﻫﺎﭘﻠﻮﺋﯿﺪ ﮔﯿﺎه داﻧﻪ روﻏﻨﯽ ﮐﺎﻣﻠﯿﻨﺎ ﺑﺎ اﺳﺘﻔﺎده از دﺳﺘﮕﺎه ﮐﺮوﻣﺎﺗﻮﮔﺮاﻓﯽ ﮔﺎزي ﺟﺮﻣﯽ )GC-Mass ﭘﺮداﺧﺘﻪ ﺷ.ﺪ ﺑﺪﯾﻦ ﻣﻨﻈﻮر، آزﻣﺎﯾﺸﯽ در ﻗﺎﻟﺐ ﻃﺮح ﺑﻠﻮك ﻫﺎي ﮐﺎﻣﻞ ﺗﺼﺎدﻓﯽ ﺑﺎ ﺳﻪ ﺗﮑﺮار اﻧﺠﺎم ﺷ.ﺪ ﻧﺘﺎﯾﺞ ﻧﺸﺎن داد ﮐﻪ ﺑﯿﻦ ﻻﯾﻦ ﻫﺎ ﺗﻔﺎوت ﻣﻌﻨﯽدار و ﺗﻨﻮع زﯾﺎدي وﺟﻮد داﺷﺖ. در اﯾﻦ ﺑﯿﻦ، ﻻﯾﻦ ﺷﻤﺎره 25 داراي ﺑﯿﺸﺘﺮﯾﻦ درﺻﺪ روﻏﻦ داﻧﻪ )41/90 %(، ﺑﻮد. اﺛﺮ ژﻧﻮﺗﯿﭗ ﺑﺮ درﺻﺪ روﻏﻦ و ﻣﯿﺰان اﺳﯿﺪﻫﺎي ﭼﺮب اﺷﺒﺎع 18:0 C20:2 C18:3 C 18:2 C24:1 C16:1 C 20:0 C ﻣﻌﻨﯽ دار ﺑ.ﻮد ﻫﻤﭽﻨﯿﻦ ﺑﯿﻦ اﮐﺜﺮ اﺳﯿﺪﻫﺎي ﭼﺮب ﻫﻤﺒﺴﺘﮕﯽ ﻣﻌﻨﯽ داري وﺟﻮد داﺷﺖ. اﮐﺜﺮ ﺻﻔﺎت ﺑﯿﻮﺷﯿﻤﯿﺎﯾﯽ داراي وراﺛﺖ ﭘﺬﯾﺮي، ﭘﯿﺸﺮﻓﺖ و ﺑﻬﺮه ژﻧﺘﯿﮑﯽ ﻧﺴﺒﺘﺎً ﺑﺎﻻﯾﯽ ﻧﯿﺰ ﺑﻮدﻧﺪ.

In this study, profiling, isolation and characterization of various valuable fatty acids and their genetic advance and genetic gain were investigated in 35 doubled haploid lines of camelina using GC-mass. For this purpose, an experiment was conducted using randomized complete block design with three replications. The results showed that there was a significant difference and diversity among the lines. Line 25 had the highest percentage of seed oil (41.90%). Genotype were different in oil percentage and saturated fatty acids content were significant for C18:0, C20:0, C16:1, C24:1, C18:2, C18:3 and C20:2 fatty acids. There was also significant correlation between most fatty acids. Most biochemical traits also had relatively high heritability, genetic advance, and genetic gain.