ﺑﯿﺎن ژن ﻫﺎي ﮐﻠﯿﺪي ﺑﯿﻮﺳﻨﺘﺰي ﻣﻮﻧﻮﺗﺮﭘﻦ ﻫﺎي ﺑﺎ ارزش در ﮔﯿﺎه داروﯾﯽ اﺳﻄﻮﺧﻮدوس ﺗﺤﺖ ﺗﻨﺶ ﭘﺮﺗﻮ ﻓﺮاﺑﻨﻔﺶ B

Expression of key genes involved in the biosynthesis of valuable monoterpenes in Lavender (Lavandula angustifolia) under UV-B stress

اﺳﻄﻮﺧﻮدوس ﯾﮏ ﮔﯿﺎه داروﯾﯽ ﺑﺎ ارزش اﺳﺖ ﮐﻪ ﻋﻼوه ﺑﺮ ﻣﺼﺎرف درﻣﺎﻧﯽ ﺑﻪ دﻟﯿﻞ ﺑﻮي ﺑﺴﯿﺎر ﻣﻄﺒﻮﻋﯽ ﮐﻪ دارد ﻣﻮرد ﺗﻮﺟﻪ ﺻﻨﺎﯾﻊ آراﯾﺸﯽ و ﺑﻬﺪاﺷﺘﯽ ﻧﯿﺰ ﻣﯽ ﺑﺎﺷﺪ. اﯾﻦ ﻧﻘﺶﻫﺎ ﺑﻪ دﻟﯿﻞ داﺷﺘﻦ ﺗﺮﮐﯿﺒﺎت ﺛﺎﻧﻮﯾﻪ ﻣﻬﻤﯽ ﻣﺎﻧﻨﺪ ﻣﻮﻧﻮﺗﺮﭘﻦ ﻫﺎ و ﺳﺰﮐﻮﺋﯿﺮﺗﺮﭘﻨﻬﺎ ﻣﯽ ﺑﺎﺷﺪ. ﻧﻈﺮ ﺑﻪ اﯾﻨﮑﻪ اﯾﻦ ﺗﺮﮐﯿﺒﺎت از ﻃﺮﯾﻖ ﻣﺴﯿﺮﻫﺎي ﺑﯿﻮﺳﻨﺘﺰي ﻧﺴﺒﺘﺎ ﻃﻮﻻﻧﯽ و ﭘﯿﭽﯿﺪه اي ﺗﻮﻟﯿﺪ ﻣﯽ ﺷﻮﻧﺪ، ﺑﺮاي اﻓﺰاﯾﺶ اﯾﻦ ﺗﺮﮐﯿﺒﺎت و ﺗﻨﻈﯿﻢ ﮐﻨﺘﺮل آﻧﻬﺎ ﻣﻄﺎﻟﻌﻪ ژﻧﻬﺎي ﮐﻠﯿﺪي اﯾﻦ ﻣﺴﯿﺮﻫﺎ ﺿﺮوري ﺑﻪ ﻧﻈﺮ ﻣﯽ رﺳﺪ. در اﯾﻦ ﺗﺤﻘﯿﻖ ﺑﺮاي اوﻟﯿﻦ ﺑﺎر ﺑﯿﺎن ﺳﻪ ژن ﮐﻠﯿﺪي ﻣﺴﯿﺮ ﺑﯿﻮﺳﻨﺘﺰي ﺗﺤﺖ ﺗﺎﺛﯿﺮ اﺷﻌﻪ UV-B ﻣﻄﺎﻟﻌﻪ ﺷ.ﺪ ژﻧﻬﺎي ﻓﻨﭽﻮل ﺳﻨﺘﺎز )FENS(، ﺑﺮﻧﺌﻮل ﺳﻨﺘﺎز )BRS(، ﺗﺮاﻧﺲ آﻟﻔﺎ ﺑﺮﮔﺎﻣﻮﺗﻦ ﺳﻨﺘﺎز )TABS( ﺑﻪ ﻋﻨﻮان ژﻧﻬﺎي ﮐﻠﯿﺪي ﺑﯿﻮﺳﻨﺘﺰ ﻣﻮﻧﻮﺗﺮﭘﻨﻬﺎ ﺗﺤﺖ ﺗﯿﻤﺎر اﺷﻌﻪ UV-B ﻗﺒﻞ و ﺑﻌﺪ از ﮔﻞ دﻫﯽ آﻧﺎﻟﯿﺰ ﺷﺪﻧﺪ و اﯾﻦ ژﻧﻬﺎ اﻓﺰاﯾﺶ ﺑﯿﺎن ﻣﻌﻨﯽ داري را در ﺳﻄﺢ روﻧﻮﺷﺖ ﻧﺸﺎن دادﻧ.ﺪ ﺑﺎ ﺗﻮﺟﻪ ﺑﻪ اﯾﻨﮑﻪ اﻓﺰاﯾﺶ ﺑﯿﺎن ژﻧﻬﺎ در ﻣﺴﯿﺮ ﺑﯿﻮﺳﻨﺘﺰي ﺑﺎ اﻓﺰاﯾﺶ ﺗﺮﮐﯿﺒﺎت ﺛﺎﻧﻮﯾﻪ در ﮔﯿﺎﻫﺎن ﻣﺮﺗﺒﻂ اﺳﺖ، ﺑﻨﺎﺑﺮاﯾﻦ اﺣﺘﻤﺎﻻ ﺗﺤﺖ ﺗﻨﺶ اﺷﻪﻌ UV-B اﻓﺰاﯾﺶ ﺑﯿﺸﺘﺮ ﺗﻮﻟﯿﺪ ﺗﺮﮐﯿﺒﺎت ﺑﺎ ارزش ﻣﻮﻧﻮﺗﺮﭘﻨﯽ اﻧﺘﻈﺎر ﻣﯽ رود. اﯾﻦ ﻧﺘﺎﯾﺞ ﻣﯽ ﺗﻮاﻧﺪ در راﺳﺘﺎي اﻓﺰاﯾﺶ ﻣﺘﺎﺑﻮﻟﯿﺘﻬﺎي ﺛﺎﻧﻮﯾﻪ ﻣﻮرد ﺑﻬﺮه ﺑﺮداري ﻗﺮار ﮔﯿﺮد.

Lavender, is a valuable medicinal plant, in addition to its therapeutic applications, has a noble odour and is of interest to the cosmetics industry. These characters are due to the important secondary metabolites such as monoterpenes and sesquiterpenes. Given that these compounds are produced through relatively long and complex biosynthetic pathways, therefore it is required to study the key genes which involved in biosynthesis in order to control their regulation and enhance production. In this study, the expression of three key genes of monoterpene biosynthetic pathway under UV-B radiation was studied. Phenchol synthase (FENS), borneol synthase (BRS) and trans-alphabergamoten synthase (TABS) were analysed as the key genes for monoterpene biosynthesis under UVB treatment before and after flowering. Results showed that these genes exhibited a significant increase in transcript expression level. Since the enhanced expression level of genes in the biosynthetic pathway is highly associated with an increase in the secondary metabolites in plants, therefore a higher increase in the production of valuable monoterpene compounds is probably expected under UV-B stress. These results can be exploited in future studies to improve monoterpene production.