ﺑﺮرﺳﯽ ﻧﻘﺶ اﻟﯿﺴﯿﺘﻮري ﻧﺎﻧﻮذرات اﮐﺴﯿﺪ ﺳﯿﻠﯿﺲ و اﮐﺴﯿﺪ آﻟﻮﻣﯿﻨﯿﻮم ﺑﺮ ﺗﻐﯿﯿﺮات ﺗﺮﮐﯿﺒﺎت ﺛﺎﻧﻮﯾﻪ و ﻣﺤﺘﻮاي ﻣﻮﺳﯿﻼژ در ﮐﺸﺖ درون ﺷﯿﺸﻪ اي ﮔﯿﺎه رﯾﺤﺎن Ocimum basilicum L

Evaluation of the elicitory role of silica and aluminum oxide nanoparticles on changes of secondary metabolites and mucilage content in vitro culture of basil (Ocimum basilicum L.)

ﺑﻪ ﻣﻨﻈﻮر ﺑﺮرﺳﯽ ﺗﺄﺛﯿﺮ ﻧﺎﻧﻮ ذرات ﻓﻠﺰي ﺑﺮ ﻣﯿﺰان ﺗﻐﯿﯿﺮات ﻓﻼوﻧﻮل ﮐﻞ و ﻓﻼوﻧﻮﺋﯿﺪ ﮐﻞ و ﻣﺤﺘﻮاي ﻣﻮﺳﯿﻼژ در ﮐﺸﺖ درون ﺷﯿﺸﻪ اي رﯾﺤﺎن، آزﻣﺎﯾﺸﯽ ﺑﻪ ﺻﻮرت ﻓﺎﮐﺘﻮرﯾﻞ در ﻗﺎﻟﺐ ﻃﺮح ﮐﺎﻣﻼً ﺗﺼﺎدﻓﯽ ﺑﺎ ﺳﻪ ﺗﮑﺮار اﺟﺮا ﮔﺮدﯾﺪ. ﻣﺤﯿﻂ ﮐﺸﺖ MS ﺣﺎوي اﮐﺴﯿﺪ ﺳﯿﻠﯿﺲ و اﮐﺴﯿﺪ آﻟﻮﻣﯿﻨﯿﻮم ﻫﺮ ﯾﮏ در ﻏﻠﻈﺖ ﻫﺎي 0، 5 و 10 ﻣﯿﻠﯽ ﮔﺮم در ﻟﯿﺘﺮ اﺳﺘﻔﺎده ﺷﺪ.ﻣﯿﺰان ﻓﻼوﻧﻮل ﮐﻞ، ﻣﯿﺰان ﻓﻼوﻧﻮﺋﯿﺪ ﮐﻞ، ﻣﯿﺰان ﻣﻮﺳﯿﻼژ، ﻗﻄﺮ اوﻟﯿﻪ ﮐﺎﻟﻮس، ﻗﻄﺮ ﺛﺎﻧﻮﯾﻪ ﮐﺎﻟﻮس، وزن ﺗﺮ ﮐﺎﻟﻮس، وزن ﺧﺸﮏ ﮐﺎﻟﻮس، ﺳﺮﻋﺖ رﺷﺪ ﮐﺎﻟﻮس و ﻣﺤﺘﻮاي آب ﻧﺴﺒﯽ ﮐﺎﻟﻮس اﻧﺪازه ﮔﯿﺮي ﺷﺪ. ﺗﺠﺰﯾﻪ وارﯾﺎﻧﺲ داده ﻫﺎ ﺗﻐﯿﯿﺮات ﻣﻌﻨﯽ داري را در اﮐﺜﺮ ﺻﻔﺎت ﻣﻮرد ﻣﻄﺎﻟﻌﻪ ﻧﺸﺎن داد. ﻣﻄﺎﺑﻖ ﻧﺘﺎﯾﺞ ﻣﻘﺎﯾﺴﻪ ﻣﯿﺎﻧﮕﯿﻦ ﻫﺎ،. ﺗﯿﻤﺎر ﺷﺎﻫﺪ ﺑﯿﺸﺘﺮﯾﻦ ﻣﻘﺎدﯾﺮ ﻓﻼوﻧﻮل و ﻓﻼوﻧﻮﺋﯿﺪ ﮐﻞ و ﺗﯿﻤﺎر 8 )ﻏﻠﻈﺖ 10 ﻣﯿﻠﯽ ﮔﺮم در ﻟﯿﺘﺮ دي اﮐﺴﯿﺪ ﺳﯿﻠﯿﮑﻮن و 5 ﻣﯿﻠﯽ ﮔﺮم در ﻟﯿﺘﺮ اﮐﺴﯿﺪ آﻟﻮﻣﯿﻨﯿﻮم( و ﺗﯿﻤﺎر 5 )ﻏﻠﻈﺖ 10 ﻣﯿﻠﯽ ﮔﺮم در ﻟﯿﺘﺮ از ﻫﺮﯾﮏ از اﯾﻦ دو ﺗﺮﮐﯿﺐ( ﺑﻪ ﺗﺮﺗﯿﺐ ﮐﻤﺘﺮﯾﻦ ﻣﻘﺎدﯾﺮ ﻓﻼوﻧﻮل و ﻓﻼوﻧﻮﺋﯿﺪ ﮐﻞ را داﺷﺘﻨﺪ. در ﮐﻞ ﻧﺘﺎﯾﺞ ﺑﯿﺎﻧﮕﺮ ﻋﺪم ﺗﺄﺛﯿﺮ ﻣﺜﺒﺖ دو ﻧﺎﻧﻮ اﻟﯿﺴﯿﺘﻮر اﮐﺴﯿﺪ ﺳﯿﻠﯿﺲ و اﮐﺴﯿﺪ آﻟﻮﻣﯿﻨﯿﻮم ﺑﺮ ﻣﻘﺎدﯾﺮ ﻓﻼوﻧﻮل و ﻓﻼوﻧﻮﯾﯿﺪ ﮐﻞ در رﯾﺤﺎن ﺑﻮد. ﺑﺎ ﮐﺎرﺑﺮد ﻏﻠﻈﺖ 5 ﻣﯿﻠﯽ ﮔﺮم در ﻟﯿﺘﺮ اﮐﺴﯿﺪ آﻟﻮﻣﯿﻨﯿﻮم )ﺗﯿﻤﺎر 2( ﺑﯿﺸﺘﺮﯾﻦ ﻣﯿﺰان ﻣﻮﺳﯿﻼژ ﺗﻮﻟﯿﺪ ﺷﺪ و ﮐﺎرﺑﺮد ﺳﺎﯾﺮ ﻏﻠﻈﺖ ﻫﺎي اﯾﻦ دو ﻧﺎﻧﻮ اﻟﯿﺴﯿﺘﻮر ﺻﻔﺖ ﻣﺬﮐﻮر را ﮐﺎﻫﺶ داد.

In order to investigate the effect of nanoparticles on the amount of total Flavenol , Flavenoid and mucilage content in basil (Ocimum basilicum L.) , a factorial experiment was conducted in a completely randomized design with three replications in MS medium containing Silicon oxide and Aluminum oxide were used at concentrations of 0,5 and 10 (mg/l). Total Flavenol content, total Flavenoid content, mucilage rate, primary callus diameter, secondary callus diameter, fresh callus weight, dry callus weight , callus growth rate and relative callus water content were measured. Analysis of variance showed significant changes in most of the studied traits. According to the results of the means comparision, control treatment had the highest values of total Flavonol and Flavenoid and treatment number 8 ( 10 mg/l Silicon oxide and 5 mg/l Aluminum oxide), and treatment number 5 ( 10 mg/l Silicon oxide and 10 mg/l Aluminum oxide), respectively. In general, the results showed no positive effect of Silicon oxide and Aluminum oxide on total Flavenol and Flavenoid in basil. Using 5 mg/l Aluminum oxide produced the highest amount of mucilage.