Author/Authors :
Ashrafosadat ،Hatamian-Zarmi Chemical Engineering Department - Faculty of Engineering - Tarbiat Modares University - Tehran, Iran , Abbas ،Shojaosadati Chemical Engineering Department - Faculty of Engineering - Tarbiat Modares University - Tehran, Iran , Ebrahim ،Vasheghani-Farahani Chemical Engineering Department - Faculty of Engineering - Tarbiat Modares University - Tehran, Iran , Saman ،Hosseinkhani Faculty of Basic Sciences - Tarbiat Modares University - Tehran, Iran
چكيده لاتين :
A bacterial strain, designated TMU56, was isolated from soil contaminated with electrical transformer fluid for over 35
years. The isolate was identified as Pseudomonas aeroginosa with respect to its 16S rDNA sequence. This strain was
found to grow on biphenyl, benzoate, naphthalene, 2-chlorobenzoic acid, 4- chlorobenzoic acid. The capability of this
strain to degrade nine polychlorinated biphenyls in liquid medium, was evaluated. This is the first reported
representative of the genus Pseudomonas capable of growing on 2,4,4’-triCB, 2,2’,5,5’-tetraCB, 2,2’,4,5,5’-pentaCB
and 2,2’,4,4’,5,5’-hexaCB as a sole carbon source. Washed benzoate-grown cells were able to degrade 100% of 2-CB ;
4-CB and 2,5-diCB after 2 days of incubation, and 78%, 89%, 64%, 75%, 69% and 56% of 4,4’-diCB; 2,4-diCB; 2,4,
4’-triCB; 2,2’,5,5’-tetraCB; 2,2’,4,5,5’-pentaCB and 2,2’,4,4’,5,5’-hexaCB, respectively after 4 days of incubation.The
position of the chlorine substituents on the rings strongly influenced the degradation of polychlorinated biphenyls by
Pseudomonas aeroginosa TMU56.
