شماره ركورد كنفرانس :
5048
عنوان مقاله :
Development an efficient method for over-production of human granulocyte colony stimulating factor (GCSF) by HCDC of recombinant E. coli
Author/Authors :
Valiollah ،Babaeipour Malek Ashtar University of Technology, Tehran, Iran , Zahra ،sahebnazar Malek Ashtar University of Technology, Tehran, Iran
كليدواژه :
rhGCSF , fed-batch , overall productivity , E. coli , specific growth rate
عنوان كنفرانس :
ششمين كنگره بين المللي مهندسي شيمي
چكيده فارسي :
يك فرآيند ساده و با كارايي بالاي تخمير غيرمداوم همراه با خوراك دهي با استفاده از يك راهبرد خوراكدهي با سرعت رشد ويژه متغير اصلاح شده براي كشت با تراكم سلولي بالاي اشريشياكلي نوتركيب BL21 (DE3) بيان كننده فاكتور محركه رشد گرانولوسيتي (GCSF) توسعه داده شد. سرعت خوراك دهي به نحويي تنظيم شد كه سرعت رشد ويژه در طول كشت غيرمداوم همراه با خوراك دهي حداكثر باشد. در اين روش سرعت رشد ويژه از مقدار بيشينه 0,55h-1 در شروع خوراك دهي به h-1 0,4 در زمان القاء تغيير داده شد.
چكيده لاتين :
A simple and high efficient fed-batch process was developed using a modified variable specific growth rate feeding strategy for high cell density cultivation of Escherichia coli BL21 (DE3) expressing human granulocyte colony stimulating factor (GCSF). The feeding rate was adjusted to achieve the maximum attainable specific growth rate during fed-batch cultivation. In this method, specific growth rate was changed from a maximum value of 0.55 h-1 at the beginning of feeding to 0.4 h-1 at induction time.
The final concentration of biomass and rh-GCSF was reached to 126 g(DCW)/l and 23 g/l after 17.5 h , also the specific yield and overall productivity of rh-GCSF were obtained 183 mg (rh-GCSF) /g DCW and 1.3 g(rh-GCSF) l-1 h-1, respectively. According to available data this is one of the highest specific yield and productivity that have been reported for recombinant proteins production yet.
