شماره ركورد كنفرانس :
5048
عنوان مقاله :
OPTIMIZATION OF PURIFICATION OF RECOMBINANT EXTRACELLULAR XYLANASE USING AQUEOUS TWO-PHASE SYSTEM
Author/Authors :
Farshad ،Rahimpour Department of Biotechnology - Center of Chemistry and Chemical Engineering - Lund University، Lund, Sweden , Gashaw ،Mamo Department of Biotechnology - Center of Chemistry and Chemical Engineering - Lund University، Lund, Sweden , Rajni ،Hatti-Kaul Department of Biotechnology - Center of Chemistry and Chemical Engineering - Lund University، Lund, Sweden
كليدواژه :
extracellular xylanase , PEG-phosphate aqueous two-phase systems , central composition design , Purification , partitioning
عنوان كنفرانس :
ششمين كنگره بين المللي مهندسي شيمي
چكيده لاتين :
A PEG- phosphate aqueous two-phase system was used for purification of
extracellular recombinant xylanase. Influence of the different factors affecting the partitioning of
recombinant xylanase and contaminant proteins, like pH, polyethylene glycol (PEG) molecular
weight, PEG and phase forming salt and NaCl concentration were studied using response surface
methodology. After partitioning of xylanase, specific activity of xylanase was increased 6.71
times, and the enzyme yield of 82% was obtained.
