بررسي رفتار زيست فعالي و زيست تخريب پذيري نانوذرات ويتلاكيت (Ca18Mg2(HPO4)2(PO4)12) براي كاربرد ترميم بافت هاي سخت بدن

Evaluation of bioactivity and biodegradability of Whitlockite nanoparticles (Ca18Mg2(HPO4)2(PO4)12) for bone regeneration

از گذشته هاي دور ترميم و بازسازي نقايص و شكستگي هاي بافت هاي سخت بدن، يك چالش بزرگ براي دانشمندان فعال در حوزه ترميم استخوان بوده است. يكي از مهم‌ترين روش هاي درمان نقايص استخواني، پيوند آلوتروپ استخوان با استفاده از سراميك هاي زيستي نظير ويتلاكيت مي‌باشد. ويتلاكيت با فرمول شيميايي (Ca18Mg2(HPO4)2(PO4)12) به عنوان دومين جزؤ معدني فراوان موجود در بافت هاي سخت بدن، خواص شيميايي، ساختاري و زيستي بسيار نزديكي به استخوان طبيعي بدن دارد. بطوريكه حدود 26 تا 58 درصد وزني از بافت استخوان هاي بدن را به خود اختصاص مي‌دهد.

Treatment and repair of bone defects, has been a major challenge for bone regeneration activists for a long time. In treating bone defects, using a proper bone graft is essential. Among various kinds of bone grafts, bio ceramics alloplastic grafts are considered, owing to their excellent non-toxicity, non- allergenicity, anti-inflammatory, biocompatibility and bio-functionality. The bio-ceramics like Whitlockite (Ca18Mg2(HPO4)2(PO4)12) possess same chemical, structural and biological properties with human bones, have been widely used as bone substitutes. It is the second most abundant mineral in living bone, which can contain 26-58 wt% of bone tissues. In this study, we investigated the biocompatibility, bioactivity and biodegradability of WH nano powders, by immersing samples in simulated body fluid (SBF) for 3,7,14 and 21 days. The obtained samples were characterized using X-ray Diffraction (XRD), Fourier transform infared spectroscopy (FT-IR) and inductively coupled plasma -optical emission spectrometry (ICP-OES). Results indicated that, WH nano powders had significantly biocompatibility and biodegradability due to formation of Ca3(PO4)2 ,(Ca,Mg)3(PO4)2 and Whitlockite on surface of WH samples. Additionally, the WH nano powders exhibited excellent mineralization ability in vitro with continuously released Ca2+, Mg2+ and HPO42- when immersed in (SBF). Furthermore, the pH changes of both samples were in the range of natural biological pH of the human body, which indicates the appropriate biocompatibility of the samples. Keywords: Whitlockite, nano particles, bioactive, biodegradable, bone regeneration, bone graft Preferred symposium: Bioactive and Biodegradable Ceramics