شماره ركورد كنفرانس :
5115
عنوان مقاله :
Evaluating toxic effects of exopolysaccharide from a Vibrio species on human gastric and colon carcinoma cells in vitro
پديدآورندگان :
Ebrahimi Ensie Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran , Khoobanfara Haniyeh Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran , Behnam Rassouli Fateme Novel Diagnostics and Therapeutics Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran , Mashreghi Mansour Industrial Biotechnology Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad,Mashhad, Iran /Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran
تعداد صفحه :
4
كليدواژه :
Vibrio# exopolysaccharide# Gastric carcinoma# Colon carcinoma# Toxic effects
سال انتشار :
1400
عنوان كنفرانس :
هفدهمين كنگره ملي مهندسي شيمي ايران
زبان مدرك :
انگليسي
چكيده فارسي :
Gastric and colon carcinomas are among the most prevalent cancers with high mortality rates worldwide, and therefore, introduction of new anticancer agents has been widely considered for their treatment. The aim of present study was to determine cytotoxicity of exopolysaccharide (EPS) derived from a Vibrio species on human gastric and colon cancer cells. To do so, after isolation of EPS from a Vibrio strain VB23, MKN45 and LoVo cells were treated with 25, 50, 100 and 200 µg/ml of VB23 EPS. Upon 24 and 48 h, cell viability was evaluated by resazurin as a colorimetric assay. Results of viability assay indicated that 200 µg/ml of VB23 EPS induced highest toxic effects on MMKN45 cells after 24 h. However, 100 µg/ml VB23 EPS induced highest cytotoxicity on LoVo cells after 24 h. Accordingly, obtained results revealed that cytotoxic effects of VB23 EPS were time- and cell type-dependent.
كشور :
ايران
لينک به اين مدرک :
بازگشت