پديدآورندگان :
Valizadeh Maryam Electroanalytical Chemistry Laboratory, Department of Chemistry, Faculty of Sciences, Azarbaijan Shahid Madani University, Tabriz 53714-161, Iran , Khalili Sepideh Electroanalytical Chemistry Laboratory, Department of Chemistry, Faculty of Sciences, Azarbaijan Shahid Madani University, Tabriz 53714-161, Iran , Mohammad-Gholipour-Rezaei Ehsan Electroanalytical Chemistry Laboratory, Department of Chemistry, Faculty of Sciences, Azarbaijan Shahid Madani University, Tabriz 53714-161, Iran , Pashazadeh Ali Electroanalytical Chemistry Laboratory, Department of Chemistry, Faculty of Sciences, Azarbaijan Shahid Madani University, Tabriz 53714-161, Iran , Pashazadeh Sara Electroanalytical Chemistry Laboratory, Department of Chemistry, Faculty of Sciences, Azarbaijan Shahid Madani University, Tabriz 53714-161, Iran , Habibi Biuck Electroanalytical Chemistry Laboratory, Department of Chemistry, Faculty of Sciences, Azarbaijan Shahid Madani University, Tabriz 53714-161, Iran
كليدواژه :
Antitubercular drug , Zeolitic imidazolate framework (ZIF , 8) , Electrochemical reduced graphene oxide , Isoniazid , Electrochemical sensor
چكيده فارسي :
In recent years, the sensitive and selective identification of drugs used in medical applications for the treatment of tuberculosis is of great importance in biomedical and pharmaceutical research [1]. Isoniazid (INZ) is found to be an important antitubercular drug for anticipation and diagnosis of pulmonary tuberculosis [2,3]. In the present investigation a new electrochemical sensor based on Copper/Zeolitic Imidazolate Framework (ZIF) −8/ Electrochemical reduced graphene oxide (Cu/ZIF-8/ErGO) for detection of isoniazid (INZ) has been developed. The morphological, structural, and electro-chemical properties of the composite were well characterized. The application of the screen-printed carbon electrode modified with Cu/ZIF-8/ErGO for the detection of antibiotic drug INZ by employing voltammetric and differential pulse voltammetry (DPV) techniques. The composite served as an outstanding electron transfer mediator for INZ oxidation. Under optimum conditions, the modified electrode showed a distinct response for voltammetric and DPVs analyses toward INZ detection, yielding low detection limits of 5.54 nM (with accessible linear ranges from 0.025 to 250 μM). The calculated charge-transfer rate constant (ks) was 2.87×10−6 cm s−1, while the electrochemical active surface area was estimated to be 0.157 cm2. The fabricated sensor exhibited good selectivity, long-term stability, and high reproducibility. The Cu/ZIF-8/ErGO/GCE were evaluated toward the sensing of INZ in human serum, urine and tablet samples. The results presented in this paper demonstrate that Cu/ZIF-8/ErGO/GCE sensing platforms provide a potential candidate for the accurate determination of INZ within human fluids and pharmaceutical formulations, which confirmed its promising applicability in medical and biomedical applications.
