Green-synthesized re-generable nanocomposite for rapid fluorescent biosensing of pathogens

Early detection and effective treatment of pathogens is of global health urgent demand [1]. A rapid and sensitive detection assay using synthesized magnetic Ag/Fe3O4/Chitosan (Ag@IO@CS) nanocomposite, was developed for efficient removal, preconcentration and fluorometric determination of trace amounts of gram negative bacteria via SPE method. Cinnamon as a bio reluctant and capping agent was used in the green synthesis procedure [2]. Prepared nanocomposite was characterized by HPTEM, FTIR and XRD measurements. The fluorescence intensity gradually increased by the prepared nanosensor as E. coli concentration increased from 1 to 104 CFU mL−1 (Fig. 1). Herein we utilized the robust design parameter optimization in the laboratory experiment for effective sensing of bacteria. A 54 design using central composite (CCD) based response surface methodology (RSM) was applied to accommodate the four significant factors as pH, solvent, time of adsorption and time of desorption of bacteria (Fig.2). All in all, preference physical and chemical properties of the prepared nanocomposite besides magnetic separation capability and simultaneous optimization advantages make this nanobiosensor excellent scaffolds for the construction of the unique probe for gram negative E. coli pathogen.