پديدآورندگان :
Noori Fariba Student Research Committee, Fasa University of Medical Sciences, Fasa, Iran, Department of Tissue Engineering, School of Advanced Technologies in Medicine, Fasa University of Medical Sciences, Fasa, Iran , Ghaderi Jafarbeigloo Hamidreza Student Research Committee, Fasa University of Medical Sciences, Fasa, Iran, Department of Tissue Engineering, School of Advanced Technologies in Medicine, Fasa University of Medical Sciences, Fasa, Iran , Jirehnezhadyan Mozhgan Student Research Committee, Fasa University of Medical Sciences, Fasa, Iran, Department of Tissue Engineering, School of Advanced Technologies in Medicine, Fasa University of Medical Sciences, Fasa, Iran , abpaikar Zahra Department of Tissue Engineering, School of Advanced Technologies in Medicine, Fasa University of Medical Sciences, Fasa, Iran , Safaei Mohsen Department of pharmaceutics, faculty of pharmacy, shahid sadoughi university of medical sciences, Yazd, Iran , Osanloo Mahmoud Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Fasa University of Medical Sciences, Fasa, Iran , Goodarzi Arash Department of Tissue Engineering, School of Advanced Technologies in Medicine, Fasa University of Medical Sciences, Fasa, Iran. dvm.goodarzi86@yahoo.com
چكيده فارسي :
Aim and Background: Skin plays an important role in human health and its defects can cause great problems. Thyme plant is one of the very useful herbal medicines that have various properties such as anti-inflammatory and antibacterial. Therefore, the properties of this plant have made this drug a suitable candidate for wound healing. In this study, a Shirazi thyme volatile oil nano gel (neZM) with a nanofibrous scaffold was used to investigate wound healing. Methods: Thyme nanoemulsion was prepared using the ionic gelation technique and then it was synthesized as nano gel by Hydroxypropyl Methyl Cellulose (HPMC). The size of the nano gel was measured by the DLS method. PCL-CS nanofiber scaffold was also synthesized by electrospinning method and its morphology was measured by SEM. To investigate the effect of this scaffold on wound healing, several 24 rats were in 4 groups 1. control, 2.neZM nano gel, 3.PCL-CS nanofiber+neZM nano gel, 4. PCL-CS nanofiber was examined in terms of histology (inflammation, edema, granulation tissue, and fibrotic tissue) on days 7, 14, and 21. Results and discussion: The results showed that the nano gel had a size of 216 ± 8 nm. Nanofibers synthesized by the electrospinning method also show smoothness without beads. The histological results also show that the PCL-CS+neZM group was able to significantly reduce the parameters of edema, and inflammation and increase the parameters of fibrosis and collagen deposition compared to other groups on days 3, 7, 21. Conclusion: The results of this study show that the neZM nanogel+ PCL-CS nanofiber group can effectively improve wound healing
