پديدآورندگان :
Boroumand Safieh Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Research Center for Advanced Technologies in Cardiovascular Medicine, Tehran Heart Center, Tehran University of Medical Sciences, Tehran, Iran , Sigaroudi Faraz Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran , Khani Mohammad-Mehdi Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran , Rabbani Shahram Research Center for Advanced Technologies in Cardiovascular Medicine, Tehran Heart Center, Tehran University of Medical Sciences, Tehran, Iran , Heirani-Tabasi Asieh Research Center for Advanced Technologies in Cardiovascular Medicine, Tehran Heart Center, Tehran University of Medical Sciences, Tehran, Iran , Ahmadi Parisa Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, TUMS, Italia Street, Tehran, Iran , Ahmadi Tafti Seyed Hossein Research Center for Advanced Technologies in Cardiovascular Medicine, Tehran Heart Center, Tehran University of Medical Sciences, Tehran, Iran , Ghanbari Hossein Research Center for Advanced Technologies in Cardiovascular Medicine, Tehran Heart Center, Tehran University of Medical Sciences, Tehran, Iran. Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, TUMS, Italia Street, Tehran, Iran. hghanbari@tums.ac.ir
چكيده فارسي :
Aim and Background: Acute myocardial infarction causes the heart to lose its proper function due to damaged tissue and cell environment. To recover the heart function, many studies presented different cardiac patches based on different biomaterials to support the infarcted myocardium. Here, we developed an nanofiberous cardiac patch with antioxidant and antibacterial properties. Methods: Nanofibers composite enriched with selenium nanoparticles (NFC/SeNPs) constructed by electrospinning technique. The characterization tests of FE-SEM, FTIR, Mechanical test, Anti-bacterial, Antioxidant used to evaluate morphology and properties of NFC/SeNPs. In vitro biocompatibility and cell attachment performed before in vivo evaluations of MI model. Results and discussion: The NFC/SeNPs scaffold with diameter of 648.36±259.19 nm displayed antioxidant properties by DPPH assay. In addition, antibacterial effects against Staphylococcus aureus and Escherichia coli bacteria demonstrated by viable cell count assessment. Improved cell adhesion and proliferation reported for NFC/SeNPs scaffold in comparison to SeNPs free scaffold, no cell toxicity showed by MTT assay. In myocardial infarction model of rat, the improved cardiac function and reduced post-surgical adhesion observed 28 days after surgery. Conclusion: In conclusion, NFC/SeNPs cardiac patch can be considered as a potent supportive strategy for myocardial rehabilitation after myocardial infarction.
