پديدآورندگان :
Emami Mina Department of Medical Nanotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran , Zomorodian Kamiar Department of Medical Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran , Yazdanpanah Somayeh Deprtment of Medical Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran , Derakhshan Mohammad Ali Department of Medical Nanotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran. ma_derakhshan@sums.ac.ir
چكيده فارسي :
Aim and Background: In the present study, a double-layered structure was obtained by electrospinning of pure type I collagen and collagen/PLLA/Zataria Multiflora essential oil nanofibers. Methods: The extracted essential oil was analyzed by GC–Mass Spectrometry and bi-layers were characterized by SEM, water contact angle measurements, XRD and ATR-FTIR. Results and discussion: Water contact angle and swelling studies showed hydrophilic nature and a high water absorption capacity of the nanofibers. Also, the essential oil illustrated a strong antioxidant activity with IC50: 7.4 ppm. Investigation of cumulative release of the essential oil from structures displayed a burst release in 120 minutes and then, a sustained release up to 72 h. Micro-dilution, disk diffusion and AATCC 100 assays were performed that exhibited strong antibacterial and antifungal activities. Alamar blue and live/dead assays illustrated the viability of the fibroblast cells on the obtained structures. Conclusion: double-layered nanofiber structures with strong antimicrobial, antioxidant and water absorption capabilities would be promising as potential wound dressings.
