پديدآورندگان :
Nowroozi Ghazal Department of Medical Nanotechnology, School of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran. Nano Drug Delivery Research System, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran , Hadibarhaghtalab Reyhaneh Department of Medical Nanotechnology, School of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran. Nano Drug Delivery Research System, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran , Arkan Elham Department of Medical Nanotechnology, School of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran. Nano Drug Delivery Research System, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran. elhamarkan@yahoo.com , Moradi Sajad Nano Drug Delivery Research System, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran , Shahlaei Mohsen Nano Drug Delivery Research System, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran
كليدواژه :
Keywords: Mupirocin , Nano liposome , Hydrogel , Nanofilm , Topical formulation
چكيده فارسي :
Aim and Background: Mupirocin (MP) is a topical antibiotic administrated for treatment of various wounds. However there are several challenges associated with the MP containing the poor dermal permeation and high risk of bacterial resistance. The new formulation strategies, such as nano formulations are nowadays use to overcome these complications. The aim of this study was to develop MP@liposome loaded polymeric nano film as drug carrier of MP. Methods: MP loaded liposomes was prepared by the thin film hydration method and were characterized by DLS, SEM and FTIR. The nanoparticles were then incorporated into the hydrogel consists of Polyvinyl Alcohol (5%wt) and Poly ethylene glycol (0/5%wt) cross-linked by NaOH/Na2SO4 solution. Pharmacological and physicochemical properties of final hydrogel were investigated using different analyzes. Results and discussion: Data achieved of DLS and SEM analyzes for nano liposome formulation showed 248/5 ± 1/5 nm, 0/243±0/04, -42/4±3/6 mV and 149/8±12 nm for hydrodynamic diameter, PDI, Zeta and nanoparticle size respectively. The FTIR results of the final product confirmed that the MP@liposomes are successfully loaded into the hydrogel. The result of tensile test indicated that the addition of nanoparticles improves the mechanical properties of nano film. Also a prolonged release profile of MP was observed from the as prepared nano composite. Cytotoxicity and antibiogram tests confirmed a non-toxic formulation with a high antimicrobial activity. Conclusion: According to the obtained results, the prepared nano-formulation in this study has excellent antimicrobial activity and can be used as proper topical delivery system for Mupirocin.
