بررسي اثر غلظت هاي مختلف NAA و ساكارز بر ريشه زايي درون شيشه گياه Gerbera jamesonii

The effect of Different concentrations of sucrose-NAA on rhizogenesis in vitro propagation of Gerbera jamesonii

ژربرا Bolus jamesonii يكي از محبوب ترين گياهان زينتي در جهان است و از طريق كشت بافت گياهي قابليت توليد انبوه در مدت زمان كوتاه وكيفيت مرغوب دارد. براي تكثير اين گياه نياز به محيط كشت با غلظت مشخصي از عناصر شيميايي، مواد آلي و به منبع انرژي وجود دارد. اين پژوهش با هدف تعيين غلظت مناسب NAA براي محيط كشت مورد استفاده و ارزيابي غلظت هاي مختلف ساكارز در كشت ريزنمونه هاي ژربرا اجرا شد. در اين آزمايش ريزنمونههاي كاپيتولوم دو رقم ژربرا شامل آرتيست و برليانس به منظور بررسي تأثير غلظتهاي مختلف ساكارز در دو سطح 20 و 30 گرم در ليتر و تنظيم كننده رشد NAA در دو غلظت 0 و 0/1 ميليگرم در ليتر كشت شدند. به جهت مناسبترين محيط كشت مورد بررسي قرار گرفت و نتايج اين آزمايش نشان داد استفاده از غلظت كمتر ساكارز باعث بهبود ريشه زايي ژربرا ش

Gerbera jamesonii Bolus is a widely popular ornamental plant globally, and its micropropagation technique enables the production of a large number of high-quality, true-to-type plants in a short period of time. In vitro propagation of gerbera necessitates the use of culture media supplemented with specific concentrations of minerals, organic supplements, and energy sources. The objective of this study was to determine appropriate culture medium and evaluate different concentrations of sucrose and Naphtalic Acetic Acid (NAA) for in vitro development of gerbera explants. This research was conducted with the aim of determining the appropriate concentration of NAA for the culture medium used and evaluating different concentrations of sucrose in the culture of gerbera explants. In this experiment, the capitulum explants of two gerbera cultivars, including Artist and Brilliance, were cultured in order to investigate the effect of different concentrations of sucrose at two levels of 20 and 30 grams per liter and growth regulator NAA at two concentrations of 0 and 0.1 mg per liter. It was investigated for the most suitable culture medium and the results of this experiment showed that the use of lower concentration of sucrose improved the Rhizogenesis of gerbera.