ارزيابي سيتولوژي دهانه رحم در گاو هاي انستروس پس از زايش كه با پروتوكل كوسينك درمان شده اند در مقابل گاوهاي طبيعي پس از زايمان

Cytological examination of the reproductive tract is often used to evaluate possible reproductive lesions in humans and domestic animals.Cytological method has been used to determination of the subclinical endometritis in the cattle.There was no information about cytological cervical cervix in anestrous cow. The aim of this study was to compare cervical cytology between normal postpartum and anestrus cow that treat by ovulation synchronization in heat day.This prospective cohort study was performed on ۵۰ estrous Holstein cows with various days in milk (DIM) and parity (۲-۴), in a moderate commercial dairy herd in susangerd, of Ahvaz province, southern Iran, from April to December ۲۰۲۲.Cervical mucus was aspirated with a pipette.Giemsa stain Cytological assessment determined the percent neutrophils (percentage PMN) by counting a minimum of ۱۰۰ cells at ۴۰۰× magnification,in order to provide a quantitative assessment of ratio %PMN to %epithelial cell.No significant differences were observed in ratio % PMN with %epithelial cell in smears of cows between anestrus cow that treatment by CoSynch and control in heat day.As well as, in our study the mean neutrophil percentage according to the parity of cows between the two groups done. Although the neutrophil percentage was higher in parity ۲ in the CoSynch group, this difference was not significant (p≥ ۰.۰۵).Our findingsindicate that anestrus dairy cow treated with synchronization of ovulation protocols in estrus day no difference of % PMN with %epithelial cell ratio in cytological smears