پديدآورندگان :
Salimizadeh Zahra salimzadeh.zahra@gmail.com Department of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran , Tahmasebi Enferadi Sattar frouz@nigeb.ac.ir Department of Plant Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran , Majidzadeh Tayebeh Department of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran , Cheraghi-shavi Tayebeh Department of Chemistry, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran , Mahjoubi Frouzandeh frouz@nigeb.ac.ir Department of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran
كليدواژه :
Peganum harmala , Colorectal cancer , MTT , drug resistance
چكيده فارسي :
Oxaliplatin is an effective chemotherapeutic medication for the treatment of colorectal cancer (CRC), however prolonged usage of it frequently leads to drug resistance. It is critical to develop methods for reducing CRC cell oxaliplatin resistance. Peganum harmala L. (P. harmala), is widely used in traditional medicine as an anticancer remedy. In present study, oxaliplatin-resistant HCT116 cells were treated with varying doses of aqueous extract derived from P. harmala seeds for 24 and 48 hours. The methylthiazol Tetrazolium (MTT) assay was used to determine cell viability. The analysis showed a significant relationship between the increase in the number of dead cells and the increase in the concentration of P.harmala’s aquaous extract. The cytotoxicity of P. harmala extract increased significantly from 24 h to 48 h in Oxaliplatin-resistant HCT116 cell line. These findings suggest that P.harmala’s aquaous extract, may be useful for treatment of drug-resistant colorectal cancer.
