پديدآورندگان :
Nekoueifard Effat Department of Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran/ Department of Cell Engineering, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran/ Nanotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran , Radmanesh Fatemeh Uro-Oncology Research Center, Tehran University of Medical Sciences, Tehran, Iran , Baharvand Hossein Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran/ Department of Developmental Biology, University of Science and Culture, Tehran, Iran , Mahdieh Athar Department of Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran , Sadeghi Abandansari Hamid Department of Cell Engineering, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran/ Department of Cancer Medicine, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Babol, Iran , Dinarvand Rasoul dinarvand@tums.ac.ir Department of Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran/ Nanotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
كليدواژه :
Smart nanocarrier , Reduction , sensitive , Magnetomicelle , Self assembly , Commercial product of liposomal DOX , Cellular uptake , Human breast adenocarcinoma MCF , 7 cells , Cytotoxicity
چكيده فارسي :
Researchers are trying to design a smart nanocarrier to deliver anticancer drugs to cancerous tissues that minimizes the side effects of these drugs. In the same direction, herein we fabricated a reduction-sensitive magnetomicelle consisting of two polymers, PEG and PCL, which are among the safest polymers in drug delivery systems. Reduction-sensitive magnetomicelle was fabricated by self-assembly of PCL-ss-PEG-ss-PCL, magnetic iron oxide nanoparticles (Fe3O4) and doxorubicin (DOX). The percentage of DOX loading in DOX- loaded magnetomicelle (D-LMM) was about 20%, which is acceptable compared to similar nanocarriers. The efficiency of D-LMM was compared with non-reduction sensitive control group (CD-LMM), free DOX and commercial product of liposomal DOX (LD) in vitro. Collectively we found significant differences between the D-LMM and LD with respect to cellular uptake and toxicity behavior in human breast adenocarcinoma MCF-7 cells. In addition, due to the reduction sensivity, D-LMM displayed better results than CD-LMM in the cytotoxicity and apoptosis/necrosis assays.
