Gene Expression of Hyoscyamine-6-beta hydroxylase (H6H) in Plantlets of Atropa belladonna L. Under Different Concentrations of Chromium

The tropane alkaloids hyoscyamine and scopolamine are widely used as anticholinergic drugs that act on the parasympathetic nervous system. The only source of the tropane alkaloids are Solanaceae plants such as Atropa, Datura, Duboisia, Hyoscyamus and Scopolia. In the biosynthetic pathway leading to the scopolamine, the enzyme hyoscyamine 6-β-hydroxylase (H6H; EC1.14.11.11) catalyses two consecutive steps; the hydroxylation of hyoscyamine to 6-ßhydroxyhyoscyamine, and the epoxidation of 6-ß-hydroxyhyoscyamine to scopolamine. In this study, the Plantlets of Atropa belladonna werecultured in a modified MS medium containing 0.1 mM IAA and 1mM BA, under different concentrations of Cr (0.2, 0.5 and 1 mM). After a month, roots were appeared. Then, total cellular RNA of the aerial parts (including stems and leaves) was extracted. cDNA of H6H gene was synthesized by specific primers. Gene expression was monitored by semi quantitative RT-PCR.> واژه هاي كليدي : ,,, atropa belladonna