تأثير تمرين فزاينده ورزشي بر mRNA آنزيم كاتالاز لنفوسيتي مردان فوتباليست

فعالیت جسمانی توانایی افزایش یا كاهش واكنش های ایمنی را دارد كه این بستگی به شدت و مدت فعالیت دارد. مشخص شده است كه ورزش برای سلامتی مفید می باشد اما بدن در طول ورزش رادیكال های آزاد را ت ولید می كند كه این مواد در نهایت منجر به ایجاد فشارهای اكسیاتیو می شود. رادیكال های آزاد بوسیله سیستم دفاعی آنتی اكسیدانی همچون آنزیم های سوپراكسید دیسموتاز، گلوتاتیون و كاتالاز خنثی می شوند. هدف تحقیق حاضر این بود كه تغییرات mRN كاتالاز لنفوسیتی را در فوتبالیست ها متعاقب یك وهله تمرین شدید ورزشی تعیین كند تا بر اساس آن میزان سازگاری كاتالاز در سطوح قبل از رونویسی بررسی شود.

Introduction : Physical exercise is capable of enhancing or suppressing the immune response depending on the intensity and duration of exercise. It is clear that exercise is beneficial to health but during exercise the body generates free radicals which are known to result in oxidative stress but are neutralized by an antioxidant system including SOD,GPX and CAT (catalase) enzymes . The purpose of this study was to identify changes in mRNA catalase of lymphocyte in soccer players following a single intensive exercise for survey adaption catalase in pre - transcriptional level . Methods : Ten voluntary male adult (23-25 age) pre Professional soccer players of Qom leage participated in this study. Blood samples were taken before, immediate and 3 hour after Exercise.To determine mRNA of catalase use from Real Time PCR . The research data analyzed by t-test. The statistical significant was set at p<0.05. Results: No significant differences were found in the mRNA catalase of lymphocyte immediate and 3 hour after intensive exercise . Conclusion : The induction of catalase (and other antioxidant enzyme) mRNA levels coincide with increases in oxidative damage of proteins and with attention to this study that was not a significant increase in mRNA catalase of lymphocyte in soccer players following intensive exercise can say that male soccer players may have a normal levels of lymphocyte catalase that been adaption to intensive exercise and this adaption may occur at the post- transcriptional level as no significant increases in catalase gene expression was found in this study.