Aktuğ, H Ege Üniversitesi - Tıp Fakültesi - Histoloji ve Embriyoloji AD, Turkey , Kosova, B Ege Üniversitesi - Tıp Fakültesi - Tıbbi Biyoloji Anabilim Dalı, Turkey , Yavasoğlu, A Ege Üniversitesi - Tıp Fakültesi - Histoloji ve Embriyoloji AD, Turkey , Çetintas, V B Ege Üniversitesi - Tıp Fakültesi - Tıbbi Biyoloji Anabilim Dalı, Turkey

Evaluation of caspase-3 and bcl-2 expressions in spleen tissue of streptozotocin-induced diabetic rats by immunohistochemistry and quantitative RT-PCR

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Aim : Spleen tissue is comprised of two functionally and morphologically distinct components, the red pulp and the white pulp. Aim of this study to evaluate the activated apoptotic pathways in spleen tissue of streptozotocin-induced diabetic rats. Apoptotic effects of diabetes in the generated animal model were analyzed in both components.Material and Methods: The working groups consisted of healthy controls (Group 1, n = 10) and streptozotocininduced diabetic rats (Group 2, n = 10). Cell morphology and integrity was evaluated by light microscopy usinghematoxylin and eosin staining. Apoptosis was determined by immunohistochemical and real-time quantitative polymerase chain reaction (RT-PCR) analysis of Caspase- 3 and Bcl-2.Results: All results were evaluated statistically. At the end of our study we determined that in the diabetic group fibrotic area contracted, causing distortion of the splenic contour and that the paranchymal fibrosis was sufficiently extensive to involve the marginal zone and follicular areas of the white pulp. In the diabetic group also more cells activated the process of apoptosis by increasing Caspase-3 and suppressing Bcl-2 expression when analyzed immunohistochemically. In agreement mRNA expression of Caspase-3 was higher in the diabetic group compared to that of the control group, whereas mRNA expression of Bcl-2 was significantly lower determined.Conclusion: These findings suggest that in spleen diabetes has an important influence on apoptotic pathways through Caspase-3 and Bcl-2.

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Apoptosis , Bcl , 2 , Caspase , 3 , diabetes mellitus , experimental

Ege Journal Of Medicine

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