Author/Authors :
Altuğlu, İ Ege Üniversitesi - Tıp Fakültesi - Tıbbi Mikrobiyoloji Anabilim Dalı, Turkey , Gürsel, D Ege Üniversitesi - Tıp Fakültesi - Tıbbi Mikrobiyoloji Anabilim Dalı, Turkey , Aksoy, A Ege Üniversitesi - Tıp Fakültesi - Tıbbi Mikrobiyoloji Anabilim Dalı, Turkey , Orman, M Ege Üniversitesi - Tıp Fakültesi - Biyoistatistik ve Tıbbi Bilişim Anabilim Dalı, Turkey , Erensoy, S Ege Üniversitesi - Tıp Fakültesi - Tıbbi Mikrobiyoloji Anabilim Dalı, Turkey
Title Of Article :
The importance and role of anti-HCV signal/cutoff ratio in diagnosis of hepatitis C virus infection
Abstract :
Aim: Different signal/cutoff values for different commercial kits are used to determine the samples that would predict a true antibody response for Hepatitis C Virus infection. The aim of this study is to determine a specific signal/cutoff ratio with the commercial kit that is used routinely in our laboratory that would predict a true antibody positive result 95% of the time regardless of the anti-HCV prevalance. Material and Methods: A total number of 309 anti-HCV positive samples that were tested with the architect anti-HCV assay (Abbott Laboratories, IL, USA) were reviewed retrospectively and categorized according to their signal/cutoff ratio. These samples were retested with LIA (Innogenetics N.V., Belgium). ROC-curve analysis in SPSS 17.0 statistical package programme was used for data analysis.Results: The signal/cutoff ratio 3.27 is determined as the cutoff point that predicts a true antibody positive result in 94.9% (positive predictive value 94.9 %, specificity 81.4%, sensitivity 91.2%) of the cases according to the ROC curve analysis.Conclusion: A stepwise approach is appropriate for evaluation of patients positive for anti-HCV. It is important for laboratories to determine a cutoff point to distinguish between a low and high positive Anti-HCV result. With the architect anti-HCV assay (Abbott, USA), in cases with signal/cutoff 3.27, the line immunoassay will be positive in most patients, so supplementary testing in such individuals is not needed.
NaturalLanguageKeyword :
Anti , HCV , hepatitis C , signal , cutoff ratio , diagnosis
JournalTitle :
Ege Journal Of Medicine
