Özdal Kurt, F Celal Bayar Üniversitesi - Fen Edebiyat Fakültesi - Biyoloji Bölümü, Turkey , Vatansever, H S Celal Bayar Üniversitesi - Tıp Fakültesi - Histoloji ve Embriyoloji Anabilim Dalı, Turkey , Tuğlu, İ Celal Bayar Üniversitesi - Tıp Fakültesi - Histoloji ve Embriyoloji Anabilim Dalı, Turkey , Deliloğlu Gürhan, S İ Ege Üniversitesi - Mühendislik Fakültesi - Biyomühendislik Bölümü, Turkey

The bone repair role of bone marrow stromal cells (BMSC) differentiated to osteoblast on the different implant materials

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Introduction: It has been thought that tissue engineering could be helpful in the wound healing of the bone by the bone marrow stromal cells (BMSC) on the biomaterials. In this study, the effect of differentiated BMSC on the different biomaterials for in vivo wound healing of experimental bone defect by the implantation will be investigated.Materials and Methods: Bone marrow stromal cells from mature male rat tibia were cultured on the three different (Cellulose Type I, Cellulose type II, Gelatine) biomaterials with culture medium including α-MEM, 10% foetal bovine serum, penicillin, streptomycin, amphotericin and gentamycine. Nonadhesive cells were removed by medium change and attach cells in the culture were incubated by osteoblastic medium including ascorbic acid, dexamethasone and β-glycerophosphate. The differentiated cells were determined by osteoblastic markers such as osteonectin and osteocalcin and were analyzed by vonkossa and alkalen phosphates for their mineralization and bone formation capacity. Their healing capacity was investigated by the histologic and morphometric methods.Results: The capacity of adhesion, proliferation and differentiation of bone marrow stromal cells was positively enhanced by these biomaterials and implantation of these biomaterials with differentiated cells produced better healing on the experimental bone defect.Conclusion: Understanding of the positive interactions between used biomaterials and cells will be helpful for the importance of tissue engineering on the clinical treatment which will promise a better quality of life for patients in the future.

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Bone marrow stromal cell , biomaterial , osteonectin , osteocalcin , wound healing , tissue engineering

Ege Journal Of Medicine

20