Author/Authors :
Bayram, Arzu İzmir Tepecik Eğitim ve Araştırma Hastanesi - Tıbbi Mikrobiyoloji Laboratuvarı, Turkey , Tezcan, Seda Mersin Üniversitesi - Tıp Fakültesi, Sağlık Araştırma Uygulama Hastanesi, Kan Merkezi, Turkey , Değirmenci, Aysu Ege Üniversitesi - Tıp Fakültesi Hastanesi, Kan Merkezi, Turkey , Emekdaş, Gürol Mersin Üniversitesi - Tıp Fakültesi, Sağlık Araştırma Uygulama Hastanesi, Kan Merkezi, Turkey , Turhan, Ajda Ege Üniversitesi - Tıp Fakültesi, Sağlık Araştırma Uygulama Hastanesi, Turkey , Erensoy, Selda Ege Üniversitesi - Tıp Fakültesi - Tıbbi Mikrobiyoloji Anabilim Dalı, Turkey , Aydınok, Yeşim Ege Üniversitesi - Tıp Fakültesi Hastanesi, Kan Merkezi, Turkey , Tiftik, Naci Mersin Üniversitesi - Tıp Fakültesi, Sağlık Araştırma Uygulama Hastanesi, Kan Merkezi, Turkey , Sertöz, Rüçhan Yazan Ege Üniversitesi - Tıp Fakültesi - Tıbbi Mikrobiyoloji Anabilim Dalı, Turkey
Abstract :
Aim: Hepatitis B virus (HBV) remains important among the agents of blood transmitted infections. Although increased sensitivity of serological tests, detection of HBV-DNA by nucleic acid tests (NAT) techniques, especially by the individual NAT (ID-NAT) techniques for safe blood transfusion came into question. Materials and Methods: A total of 4352 HBsAg negative volunteer-donors in ages of 18-65 years admitted to the Blood Transfusion Centers of Ege University Faculty of Medicine and Mersin University Faculty of Medicine Hospital were included in the study between April 2010 and January 2011. The samples of the donors were tested with real-time polymerase chain reaction (RT-PCR) in order to detect HBV-DNA. Results: Following HBV-DNA screening, only two positive serum results were found. Test was repeated on two positive samples with both the same and an alternative method. Repeated tests resulted negative; therefore all samples were assessed as HBV-DNA negative. In all samples of HBsAg negative donors from two different centers, HBV-DNA was found to be negative. Conclusion: The number of blood donors included in the study and low seroprevalence of the centers could be the reason for no detection of occult HBV. Turkish Red Crescent routine NAT studies that began in 2014 will give appropriate data for occult HBV in our region
NaturalLanguageKeyword :
Blood donor , Hepatitis B virus , HBV , DNA , nucleic acid tests
