Altındiş, Selma Afyon Kocatepe Üniversitesi - Uygulama Araþtirma Hastanesi - Dis Hekimligi Klinigi, Turkey , Altındiş, Mustafa Afyon Kocatepe Üniversitesi - Tip Fakültesi - Mikrobiyoloji AD, Turkey , Özdemir, Müjgan Afyon Kocatepe Üniversitesi - Tip Fakültesi - Tibbi Biyoloji ve Genetik AD, Turkey , Ellidokuz, Ender Celal Bayar Üniversitesi - Tip Fakültesi - Gastroenteroloji AD, Turkey , Uslan, İhsan Afyon Kocatepe Üniversitesi - Tip Fakültesi - Gastroenteroloji AD, Turkey , Duran, İsmet Selçuk Üniversitesi - Dis Hekimligi Fakültesi - Periodontonloji AD, Turkey , Dilek, Hüsniye Afyon Kocatepe Üniversitesi - Tip Fakültesi - Patoloji AD, Turkey

Detection of helicobacter pylori in dental plaque and gastric biopsy samples of patients whose gastric infections by real time-PCR

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Helicobacter pylori are regarded as an important pathogen playing a key role in the pathogenesis of peptic ulcer. Dental plaque has been suggested as a reservoir for H.pylori but the hypothesis that the oral microflora may be a permanent reservoir of H. pylori is still controversial. The aims of this study were to determine the presence of H. pylori DNA in the gastric antrum and dental plaque of a Turkish population by RT- PCR and to investigate the relationship between this infection and the oral hygiene index. Sixty patients attending for routine gastroscopy by dispeptic symptoms, and 40 asymptomatic subjects (control group) were evaluated. The patients dental plaque was assessed by the plaque indices of Sillness and Loe. Supragingival plaque was analysed by a RT PCR for a specific internal urease gene. Gastric antrum biopsies were taken for histological examination and RT-PCR. H.pylori was detected by histological examination in antral samples from 51 (85 %) and by RT PCR 44(73.3 %) of 60 patients, all of who had gastritis. H.pylori was also detected in dental plaque samples of 5 (8.3 %) of the 60 patients. In 4 (80 %) of these 5 patients, H.pylori was identified in the gastric biopsy. Four patients with chronic gastritis carried H. pylori in dental plaque and antral samples. Of these patients, one also had dysplasia and one had metaplasia. Only one subject in the control group was positive by PCR. There was no correlation between gastric H.pylori infection and dental hygiene, dental caries, periodontal disease or use of dentures.

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Helicobacter pylori , dental plaque , gastric biopsy , RT , PCR

Medical Journal Of Suleyman Demirel University

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