Author/Authors :
Çetin, Emel Sesli Süleyman Demirel Üniversitesi - Tip Fakültesi - Tibbi Mikrobiyoloji AD, Turkey , Aynali, Ayse Süleyman Demirel Üniversitesi - Tip Fakültesi - Tibbi Mikrobiyoloji AD, Turkey , Öztürk, Tuba Süleyman Demirel Üniversitesi - Tip Fakültesi - Tibbi Mikrobiyoloji AD, Turkey , Özseven, Ayse Gül Süleyman Demirel Üniversitesi - Tip Fakültesi - Tibbi Mikrobiyoloji AD, Turkey , Kaya, Selçuk Süleyman Demirel Üniversitesi - Tip Fakültesi - Tibbi Mikrobiyoloji AD, Turkey
Title Of Article :
Evaluation of the Bactec Mycobacteria Growth Indicator Tube 960 system with Löwenstein-Jensen medium for recovery of mycobacteria from clinical specimens
Abstract :
Objective: In this study, we aimed to evaluate the Bactec Mycobacterium Growth Indicator Tube (MGIT) 960 system and Löwenstein-Jensen (LJ) medium for recovery rates and times of mycobacteria from clinical specimens. Methods: A total of 2400 specimens (1138 sputum, 395 gastric aspirate, 237 urine, 165 pleural fluid, 139 bronchial alveolar lavages, 125 peritoneal fluid, 53 CSF, 49 abscess, 19 tissue biopsy, 17 bronchial biopsy, 16 wound, 15 bone marrow biopsy, 15 joint fluid, 13 lymph node aspirate, 4 pericardial fluid) which were sent to microbiology laboratory of Suleyman Demirel University Medical Faculty between October 2007 and September 2010 were included in this study. Results: A total of 148 (6.2%) specimens were positive for acid-fast organisms by smear, LJ culture, or MGIT 960 system. 92.6% of these were positive with MGIT 960, while 68.2% of them were positive with LJ culture. The recovery rate for mycobacteria was significantly higher and time of recovery was significantly lower with MGIT 960 than LJ culture (P .0001). Conclusion: Our findings showed that although the MGIT 960 system provided better recovery of mycobacteria from clinical specimens than did the traditional LJ culture, the presence of the specimens which were positive with LJ culture but not with MGIT 960 makes us suggest that both media types are necessary to provide the best results for the recovery of mycobacteria from clinical specimens.
NaturalLanguageKeyword :
Mycobacterium tuberculosis , Löwenstein , Jensen , MGIT 960
JournalTitle :
Medical Journal Of Suleyman Demirel University
