TOK, Rafet Batman Özel Dünya Hastanesi - Kalp ve Damar Cerrahisi Kliniği, Türkiye , UYSAL, Ayhan Fırat Üniversitesi - Tıp Fakültesi - Kalp ve Damar Cerrahisi Anabilim Dalı, Türkiye , BURMA, Oktay Fırat Üniversitesi - Tıp Fakültesi - Kalp ve Damar Cerrahisi Anabilim Dalı, Türkiye , ÖZERCAN, İbrahim Hanifi Fırat Üniversitesi - Tıp Fakültesi - Patoloji Anabilim Dalı, Türkiye

The Effects of Vasodilator Solutions on Apoptosis in Saphenous Vein with Mechanical Distention Application

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Objective: This study explores the influence of vasodilators on the apoptotic activity related to endothelial damage caused by mechanical distension of the saphenous vein. Materials and Methods: Thirty-two patients without varicose veins undergoing coronary artery bypass graft operations were divided into four equal groups. Group I (control) Normal Saline, group II Papaverine group III Verapamil and group IV Nitroglycerine solutions. Percentange of immunohistochemical reactivity, pro-apoptotic bax and kaspaz-9 and anti apoptotic bcl-2 were measured in the samples. Results: The percentage of cells which were positive for Bax (+) In Group I (Control) was 85.00 ± 6.89, Group II (Papaverine) 64.63 ± 8.73, Group III (Verapamil) 23.75 ± 4.20 and Group IV (Nitroglycerine) 22.00 ± 7.69. While there was no statistical difference between verepamil and nitroglycerin groups, they both had significantly decreased percentage of cells with proapoptotic Bax (+) compared to the control and the papaverine groups (p 0.001). Percentage of cells that were stained with the antiapoptotic BCL (+) stain were lowest in the control (2.50 ± 1.41) and the Papaverine (2.75 ± 1.28) groups and significantly higher in the Verapamil (7.25 ± 1.83) and Nitroglycerine (8.88 ± 2.30) groups (p 0.001). There was no difference between the groups with respect to Caspase-9 immunoreactivity. Conclusion: The results of this study suggest that addition of Verapamil and or Nitroglycerine to the solutions that are used in the preparation of the saphenous vein might have a beneficial effect on the reduction of the apoptotic activity.

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Apoptosis , Vasodilator , Saphenous vein

Fırat Medical Journal

99