مرکز منطقه ای اطلاع رساني علوم و فناوري - The Affects of Adriamycin Implementation to The Immunreactivity of Nesfatin-1 at Rat Heart Tissue

Abstract :

Objective: Adriamycin is a broad-spectrum anticancer drug that is used at various type of solid tumors and hematologic malignancies. The main side effects of adriamycin are heart failure and cardiomyopathy. Studies suggests that, oxidative stress plays an important role in the pathogenesis of adriamycin cardiotoxicity. Nesfatin-1 is closely associated with diabetes, obesity, anorexia nervosa, psychiatric disorders, and neurogenic diseases. It has been reported that Nesfatin -1 directly affects the myocardial performance and protects the myocardium from ischemia reperfusion injury. Material and Method In this study Wistar albino rats were used. Rats were divided into 2 groups. No application was made to control group at 14 days. To adriamycin group, 10mg/kg single dose adriamycin was injected intraperitoneally. Finally rats were decapitated and heart tissues were removed. With routine procedures, tissues were embedded in paraphine blockes. For nesfatin-1 immunreactivity ‘‘avidin-biotin-peroxidase’’ method was applied. Results In control group was observed at +3 diffusiveness in myocytes at heart tissue. In comparison with control group, nesfatin-1 immunoreactivity showed significant decrease at adriamycin group and was at +1 diffusiveness. Conclusion: As a result, rat heart tissue releases nesfatin-1, experimental implementation of adriamycin reduces nesfatin-1 immunoreactivity in rat heart tissue, and this decrease depends on myocardial damage / loss due to adriamycin.