مرکز منطقه ای اطلاع رساني علوم و فناوري - A Research on Effects of Ethyl Pyruvate on Rat Testical Tissues which Was Damaged with Cadmium

Abstract :

Objective: The present study aimed to evaluate the protective effect of Ethyl Pyruvate on cadmium induced testicular apoptosis and toxicity in rats. Material and Method: In this study 32 adult male Wistar albino rats were used. Rats are seperated into four random groups. Group 1; (n=8) control group, Group 2; (n=8) 2.5 mg/kg intraperitoneal (ip) cadmium administered rats, Group 3; (n=8) 2.5 mg/kg ip cadmium + 100 mg/kg ethyl pyruvate administered rats, Group 4; (n=8) 100 mg/kg ethyl pyruvate administered rats. Two doses of the ethyl pyruvate were administered in a 24 hours interval. Cadmium was applied 1 hour after administration of ethyl pyruvate to the groups 2 and 3 and all the subjects are killed by decapitation 24 hour after and their testis tissues were collected. Results: Germinal epithelium irregularities, epithelial cell loss in lumen, formation of the vacuoles between epithelial cells and necrotic tubules were observed in the cadmium applied group. The diameters of seminiferous tubules, tubular biopsy score of Johnsen and tissue MDA levels significantly decreased and apoptotic cell numbers significantly increased compared to control group. It was observed that ethyl pyruvate has only shown improvement in the number of apoptotic cells. Conclusion: As a result application of cadmium create very serious changes on testicular tissue and the ethyl pyruvate administration for protective purposes is not preventing this damage except improvement in the number of apoptotic cells.