Author/Authors :
KARACA, Ömür Balıkesir Üniversitesi - Tıp Fakültesi - Anatomi Anabilim Dalı, TÜRKİYE , PEKMEZ, Hıdır Fırat Üniversitesi - Tıp Fakültesi - Anatomi Anabilim Dalı, TÜRKİYE , KUŞ, Murat Abdülgani Mehmet Akif Ersoy Üniversitesi - Sağlık Yüksekokulu, TÜRKİYE , AKPOLAT, Nusret İnönü Üniversitesi - Tıp Fakültesi - Patoloji Anabilim Dalı, TÜRKİYE , ÖGETÜRK, Murat Fırat Üniversitesi - Tıp Fakültesi - Anatomi Anabilim Dalı, TÜRKİYE , KUŞ, İlter Balıkesir Üniversitesi - Tıp Fakültesi - Anatomi Anabilim Dalı, TÜRKİYE
Abstract :
Objective: Carbon tetrachloride (CCl4) is a volatile organic chemical, which causes tissue damage, especially to the liver and kidney. The potential for protective effects of melatonin on carbon tetrachloride-induced acute liver injury in rats was investigated in this work.Materials and Methods: Twenty-four male Wistar-Albino rats were randomly divided into three equal groups: Control, CCl4 and CCl4 plus melatonin (CCl4+Melatonin). Rats in CCl4 group were injected subcutaneously with CCl4 0.5 ml/kg in olive oil while rats in CCl4+Melatonin group were injected subcutaneously with CCl4 (0.5 ml/kg) plus melatonin (25 mg/kg) every other day for one month. Control rats were injected olive oil. Hepar tissues were examined immunohistochemically to detect heat shock protein 70 immunoreactivity.Results: A dense staining indicating Hsp70 immunoreaction was observed in the liver depending on the toxicity of CCl4. The liver tissue sections of rats injected with melatonin and CCl4, the HSP70 staining was detected minimal.Conclusion: As a result of these findings obtained from immunohistochemical study; ıt was detected that CCl4 induced tissue damage in the liver were prevented by the hormone melatonin.
NaturalLanguageKeyword :
Carbon tetrachloride , melatonin , liver , Hsp70
