Author/Authors :
dündar, serkan fırat üniversitesi - diş hekimliği fakültesi - periodontoloji anabilim dalı, Elazığ, Turkey , dedeoğlu, numan inönü üniversitesi - diş hekimliği fakültesi - ağız, diş ve çene radyolojisi anabilim dalı, Malatya, Turkey , talo yildirim, tuba fırat üniversitesi - diş hekimliği fakültesi - periodontoloji anabilim dalı, Elazığ, Turkey , bozoğlan, alihan fırat üniversitesi - diş hekimliği fakültesi - periodontoloji anabilim dalı, Elazığ, Turkey , çakmak, ömer afyon kocatepe üniversitesi - diş hekimliği fakültesi - ağız, diş ve çene radyolojisi anabilim dalı, Malatya, Turkey , bulmus, özgür fırat üniversitesi - sağlık bilimleri fakültesi - fizyoterapi ve rehabilitasyon bölümü, Elazığ, Turkey
Abstract :
Objective: The aim of this study is to investigate the radiological and biochemical examination of periodontal tissue destruction in ligature induced experimental periodontitis in restrain stress model. Materials and Methods: Twenty adult female Spraque Dawley rats weighing 390-450 grams were included in the study. Rats were divided in two groups; controls (n =10) and stress (n = 10). At the beginning of the study, rats weights were measured. Ligatures were placed around both left and right mandibular first molars subgingivally of the all rats in general anesthesia. Control group rats were not subjected to any additional treatment during the 28-day experimental period and stress group rats were applied restraint stress 2.5 hours per day. After a period of 28 days of experimentation, the rats were sacrificed in the deep anesthesia following weighing. Both left and right mandible of all rats were taken to determine the radiological bone destruction. In addition, cortisol levels of blood serum of all rats were determined by biochemical analysis. All datas were shown using with mean and standard deviation. Results: After the radiological analysis no statistically significant difference was not detected between controls and stress groups in mesial and distal periodontal bone destruction percentages (P 0,05). The mean weight of the stress groups is lower than controls (P 0,05). Blood cortisol values are detected as higher in stress group when compared with the controls (P 0,05). Conclusion: According to the radiological analysis, 28 day restrain stress did not effect the periodontal tissue destruction. In addition, restraint stress caused an increase in serum cortisol levels, which is a stress indicator in rats, and also weight loss, which is an other stress indicator.
NaturalLanguageKeyword :
Experimental periodontitis , restrain stress , cortisol , rat
