Author/Authors :
ÖZÇELİK, Mehtap Veteriner Kontrol ve Arastirma Enstitüsü, Turkey , KANDEMİR, Fatih Mehmet Firat Üniversitesi - Veteriner Fakültesi - Biyokimya Anabilim Dali, Turkey , ÖZDEMİR, Necmi Fırat Üniversitesi - Veteriner Fakültesi - Biyokimya Anabilim Dali, Turkey
Title Of Article :
Inhibitory Effect of Ethylene Diamine Tetra Acetic Acid (EDTA), p-Chloromercuri Benzoic Acid (p-CMBA), N-Ethyl Maleimide (NEM) on Arginase Activity of the Sheep Kidney Tissue and Kinetic of Inhibition
Abstract :
In this study, the inhibition effects of EDTA, p-CMBA, NEM on arginase activity in sheep kidney tissue were studied. Arginase activity was spectrophotometrically measured using the Tiyosemicarbazid- Diacetylmonoksim-Urea Method. Protein was determined by the method of Lowry. The kidney tissues of sheep in the present study were obtained from the Elkas sloughterhause in Elazıg. This study was carried out using 20 sample of kidney tissue. In conclusion, arginase activity in sheep kidney tissue was determined to be inhibited competative inhibition by EDTA but to be inhibited noncompetative inhibition by NEM, p-CMBA.
NaturalLanguageKeyword :
Arginase , EDTA , NEM , p , CMBA
JournalTitle :
Fırat University Veterinary Journal Of Health Sciences
