Author/Authors
ERDEM, Nevher Fırat Üniversitesi - Veteriner Fakültesi - Biyokimya Anabilim Dalı, Turkey , ERİŞİR, Mine Fırat Üniversitesi - Veteriner Fakültesi - Biyokimya Anabilim Dalı, Turkey
Title Of Article
Optimization of Liver Arginase in Rabbit and Distiribution in Tissues
شماره ركورد
18070
Abstract
Optimum conditions for liver arginase in rabbit and distribution in the tissues was investigated. In the study, the tissues of six one-year-old New Zealand rabbits were used. Its was found that preincubation temperature 65 °C, preincubation period 13 minutes, incubation period 10 minutes and optimum pH 10 for arginase in rabbit liver tissue. Enzyme achieved its highest activity at 2 mM MnCl2 concentration. Km of arginase in rabbit liver tissue for L-arginine were measured to be 1.6 mM. Consequently, it was determined that Mn^+2 ions and preincubation at 65 °C were essential for the activation of the enzyme. Among liver, kidney, heart, uterus, brain, spleen, intestine, esophagus, muscle, tongue, lung, stomach, trachea samples, the highest arginase activity was found in the liver and the lowest arginase activity was found in trachea.
From Page
81
NaturalLanguageKeyword
Rabbit , arginase , optimization
JournalTitle
Fırat University Veterinary Journal Of Health Sciences
To Page
86
JournalTitle
Fırat University Veterinary Journal Of Health Sciences
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