ÇERİBAŞI, Songül Fırat Üniversitesi - Veteriner Fakültesi - Patoloji Anabilim Dalı, Turkey , ÖZKARACA, Mustafa Elazığ Veteriner Kontrol Enstitüsü - Patoloji Laboratuvarı, Turkey , ÇERİBAŞI, Ali Osman Fırat Üniversitesi - Veteriner Fakültesi - Patoloji Anabilim Dalı, Turkey , ÖZER, Harun Fırat Üniversitesi - Veteriner Fakültesi - Patoloji Anabilim Dalı, Turkey

Immunohistochemical Detection of Peste des Petits Ruminants (PPR) Viral Antigens in Pneumonic Caprine Lungs in Elazig Region

18073

The aim of this study was to evaluate presence and distribution of Peste des Petits Ruminants (PPR) infection by immunohistochemistry in goats with pneumonia in the Elazig region. For this purpose, lungs of 889 slaughtered male hair goats were macroscopically analysed for pneumonia lesions. Among the 152 cases of pneumonia, 97 (63.82%) exhibited mild lesions, 39 (25.66%) had moderate lesions and 16 (10.53%) had severe lesions according to the extent of consolidation, and they were classified microscopically as catarrhal-purulent (n=41, 26.97%), interstitial (n=72, 47.37%), fibrinous (n=34, 22.37%), verminous (n=2, 1.32%), and pulmonary adenomatosis (n=3, 1.97%). The PPR antigens were detected in 7 cases three of which had catarrhal-purulent bronchopneumonia with mild consolidation, two had interstitial and the remaining had fibrinous bronchopneumonia with moderate consolidation by using direct immunohistochemistry on paraffine sections. Specific immunostaining related with viral antigens was found more intensely in the bronchi and the bronchiole epithelium and, also cellular exudates in the lumen. These results show that PPR viruses may be regarded as one of the aetiological agents in goat pneumonia. Furthermore, when non-specific histopathological lesions in the pneumonia with PPR origin, as evidenced in the presented study, are considered, the immunohistochemistry can be used as an alternative method to the advanced laboratory methods for diagnosis of PPR infections in goats.

99

Peste des Petits Ruminants virus , goat , pneumonia , immunohistochemistry

Fırat University Veterinary Journal Of Health Sciences

103