OZAN, Gonca Fırat University - Veteriner Fakültesi - Biyokimya Anabilim Dalı, TÜRKİYE , KAYA, Nalan Fırat Üniversitesi - Tıp Fakültesi - Histoloji - Embriyoloji Anabilim Dalı, TÜRKİYE , YILMAZ, Osman Fatih Fırat Üniversitesi - Tıp Fakültesi - Histoloji - Embriyoloji Anabilim Dalı, TÜRKİYE , ERDEM, Elif Artuklu Üniversitesi - Sağlık Yüksekokulu - Department of Ophthalmology, TÜRKİYE , OZAN, Enver Fırat Üniversitesi - Tıp Fakültesi - Histoloji - Embriyoloji Anabilim Dalı, TÜRKİYE

The Effect of Oleuropein on Ghrelin Expression in Rat Testes with Fructose- Induced Metabolic Syndrome

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We aimed to investigate the effects of oleuropein administration on ghrelin expression in rat testes tissues with fructose-induced metabolic syndrome by immunohistochemical and biochemical methods. Thirty-two male adult (8 week aged) Sprague-Dawley rats were randomly divided into four groups (n=8); Control, Meabolic syndrome, Oleuropein, Metabolic syndrome plus Oleuropein. Metabolic syndrome was induced by fructose solution 20% in drinking water, and oleuropein was administered at the dose of 10 mg/kg daily by oral gavage. After the experimental period of 60 days, the rats were decapitated, testes tissues and blood samples were taken. Serum glucose, insulin and triglyceride levels were measured. The testes samples were immunehistochemically stained using avidin-biotin-peroxidase method for the determination of ghrelin immunoreactivity. Also testes tissue malondialdehyde (MDA) levels and catalase activity were determined spectrofotometrically. Fructose consumption significantly increased serum triglyceride, insulin levels and insulin resistance. Ghrelin immunoreactivity in rat testes tissues with fructose-induced metabolic syndrome was determined as low. Oleuropein administration increased testes ghrelin levels. In comparison with control group, MDA levels increased and CAT activities decreased in metabolic syndrome group. On the other hand MDA levels were diminished and Cat activities were elevated in fructose plus oleuropein group compared to the metabolic syndrome group. These results indicate that metabolic syndrome increases oxidative stress in testicular tissue. It can be suggested that at that the dose and time of oleuropein administration can play a role as an antioxidant and protect the testes from the effects of high fructose induced metabolic syndrome.

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Metabolic syndrome , oleuropein , ghrelin , testes

Fırat University Veterinary Journal Of Health Sciences

133