Protective Effect of Ginkgo Biloba Extract 761 on Di-(2-ethylhexyl) Phthalate-Induced Testicular Toxicity in Prepubertal Rats

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In this study, the protective effect of ginkgo biloba extract 761 (EGb 761)’s on testicular damage caused analyzed by giving the same dose di-(2-ethylhexyl) phthalate (DEHP) to rats at different times was investigated. 72 Wistar Albino rats at 28 days of age were divided into groups of control, DEHP, DEHP+EGb 761 and EGb 761. On the 7th,14th and 28th days of the study, the animals were decapitated. DEHP administration was determined to cause significant decreases in testes weights, diameter of seminiferous tubules, germinal cell layer thickness, serum testosterone levels, glutathione (GSH) levels wherease it caused significant increases in malondialdehyde (MDA), glutathione peroxidase (GSH-Px) and catalase (CAT) levels, apoptotic indices along with degeneration, necrosis, atrophy, disorganization, Leydig cell hyperplasia and thickening of the basal membrane in testicular tissue when compared with the control group. EGb 761 administration provided significant improvements in disturbed oxidant/antioxidant balance, decreased testosterone concentration, induced testicular apoptosis and gradually improvements in the histopathological view of the testicular tissue in DEHP-treated rats. It was determined that DEHP is highly toxic to the testis tissue for the application duration and dose and cause to severe structural damage and EGb 761 which has antioxidant effect prevents this damage.

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Apoptosis , di , (2 , ethylhexyl) phthalate , ginkgo biloba extract 761 , testicular toxicity , TUNEL

Fırat University Veterinary Journal Of Health Sciences

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Fırat University Veterinary Journal Of Health Sciences