çiftçi, nihal gaziosmanpaşa üniversitesi - tıp fakültesi - tıbbi biyokimya anabilim dalı, Tokat, turkey , gevrek, fikret gaziosmanpaşa üniversitesi - tıp fakültesi - histoloji ve embriyoloji anabilim dalı, Tokat, turkey

Biochemical, Histopathological, Immunohistochemical Evaluation of Ischemic Preconditioning and Krill Oil Effects in Ischemia / Reperfusion Model

18236

The aim of the present study was to evaluate the protective effects of krill oil (KO) administration and ischemic preconditioning (IPC) protocol using biochemical, histopathological and immunohistochemical parameters in a rat model. Limb ischemia was achieved by clamping the femoral arteries. Fifty rats were divided into five groups (n=50). Effect of IPC strategy and drug-reinforcement protocol was evaluated together or separately in the experimental groups. Malondialdehiyde (MDA), nitric oxide (NO), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) activities were measured. Light microscopic images were supported by histological damage score findings and semiquantitative analysis findings of β-actin protein. Biochemical markers of oxidative stress were improved in KO and IPC groups compared with I/R group. The administration of IPC and KO resulted in a significant decrease in the damage score, 40% reduction of damage levels. Semiquantitative H-score analysis revealed that immunoreactivity increased in the treatment groups compared with I/R and IPC groups. It is concluded that ischemic-preconditioning protocol and pre-treatment with KO provides potent protection ischemia-reperfusion injury.

159

Skeletal muscle , femoral artery , krill oil , ischemic preconditioning

Fırat University Veterinary Journal Of Health Sciences

167