yeni, deniz afyon kocatepe university - faculty of veterinary medicine - department of reproduction and artificial insemination, Afyonkarahisar, TURKEY , avdatek, fatih afyon kocatepe university - faculty of veterinary medicine - department of reproduction and artificial insemination, Afyonkarahisar, TURKEY , gündoğan, mustafa afyon kocatepe university - faculty of veterinary medicine - department of reproduction and artificial insemination, Afyonkarahisar, TURKEY

The Effect of Boron Addition on Spermatological Parameters, Oxidative Stress and DNA Damage After Frozen-Thawed Process in Ramlic Ram Semen

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The aim of the present study was to investigate the effects of different doses of Boron added to extender on some spermatological parameters, oxidative stress, and DNA damage during post- thawed of ram semen. The ejaculate samples were collected by artificial vagina from five Ramlıç rams. After determining the primary spermatological properties, those having normospermie quality were pooled. The samples were evaluated as a mix ejaculate in the experiment and control groups. The samples were extended with Tris extender containing 5% glycerol and different Boron doses (1, 2 and 4 mM). Control group was formed with no Boron. After dilution and dosing of the samples, withdrawn in 0.25 mL straws were frozen at vapour of liquoid nitrogen. The frozen semen was thawed in a water bath at 37 ºC for 25 seconds. After thawing; motility, morphology, DNA integrity (COMET assay) and oxidative stress parameters (TAS and TOS) of frozen thawed ram sperm were determined. In the thawed semen according to subjective motility (57.00±1.528 %), 1 mM boron dose group indicated the highest value (P 0.05). Head, tail piece and TOS values were found highest in 4 mM group compared to the control group (P 0.05). In terms of DNA damage, 1 mM (34.80±1.289 AU) had the lowest value compared with the control group (48.10±3.093 AU). In terms of mitochondrial activity, the highest value was achieved in 1 mM (34.60±1.035 %) compared with the control group (26.40±1.740 %).In conclusion, it is thought that using boron might have a positive impact on energy metabolism, DNA damage in freezing, and thawing phase of spermatozoa, and therefore sperm may increase the ability of fertilization.

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Boron , DNA damage , ram semen , oxidative stress , cryopreservation

Fırat University Veterinary Journal Of Health Sciences

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