Author/Authors :
Gürbüz, Burçak Marmara Üniversitesi - Eczacilik Fakültesi - Farmasötik Mikrobiyoloji Anabilim Dali, Turkey , Gürer, Ümran Soyoğul Marmara Üniversitesi - Eczacilik Fakültesi - Farmasötik Mikrobiyoloji Anabilim Dali, Turkey , Çevik, Özge Cumhuriyet Üniversitesi - Eczacilik Fakültesi - Biyokimya Anabilim Dali, Turkey , Yalçınkaya, İrfan Sağlık Bakanlığı - Süreyyapasa Gögüs Hastaliklari ve Gögüs Cerrahisi Egitim ve Arastirma Hastanesi, Turkey , Bekiroğlu, Gülnaz Nural Marmara Üniversitesi - Tip Fakültesi - Biyoistatistik veTibbi Bilisim Anabilim Dali, Turkey , Çevikbaş, Adile Marmara Üniversitesi - Eczacilik Fakültesi - Farmasötik Mikrobiyoloji Anabilim Dali, Turkey
Title Of Article :
In vitro investigation of the effects of colistin alone with and tigecycline, imipenem and rifampicin combinations on polymorphonuclear leukocyte functions, oxidative stress, oxidant and antioxidant enzymes in patients with bronchiectasis.
Abstract :
In our study was investigated the in vitro effects of Colistin(4 μg/ml), alone with the combination of tigecycline(0.87 μg/ml), imipenem (30 μg/ml) and rifampicin (7 μg/ml) at therapeutic concentrations and malondialdehyde (MDA) level, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and myeloperoxidase (MPO) activity on polymorphonuclear leukocyte (PMN) functions (phagocytic and intracellular killing activity) of patients with bronchiectasis. PMNLs were isolated from venous blood by Ficoll-hypaque gradient centrifugation method. Phagocytic activity and intracellular killing activity were assayed by modified Alexander’s method. MDA levels were determinated by Beuge’s method, SOD, GSH-Px and MPO activities were assayed by Sun’s, Paglia and Valentine’s and Hillegas’s, methods respectively. All results are statistically evaluated. Phagocytic (p 0.0001), and intracellular killing activity (p=0.0113, p=0.0008, p=0.0014, p=0.0036)in the PMN of patients with bronchiectasis significantly increased when compared to the control. Enzyme activity (SOD, GSH-Px and MPO) and MDA level significantly decreased (p 0.001) in patients with bronchiectasis when compared to the control.
NaturalLanguageKeyword :
Antioxidants , bronchiectasis , phagocytosis , oxidative stres
JournalTitle :
Journal of Research in Pharmacy
