Gökdeniz, Remzi Inonu University - Medical Faculty - Department of Obstetrics and Gynecology, Turkey , Ovayolu, Ali Joseph’s Hospital - Department of Obstetrics and Gynecology, USA , Garfield, Robert E. Joseph’s Hospital - Department of Obstetrics and Gynecology, USA

Progesterone Inhibits Human Myometrial Contractions by its Action on Membrane Receptors

23792

Purpose: The mechanisms for myometrial inhibition are still being investigated. The aim of this article is to examine mechanisms of progesterone (P4) inhibition of uterine contractility. Methods: Prospective study Tertiary care center at St. Joseph’s Hospital and at Maricopa Hospital, Phoenix, AZ and research center in Arizona, USA. During 2010-2011 24 women were given birth by cesarean section. Uterine tissues from women (n=24) at term were suspended in organ chambers and exposed to various agents. Contractility was recorded and compared before and after addition of agents. Tissues were treated with P4 alone, a progestin (R5020) with low affinity to the progesterone membrane receptor (mPR), or a non-sex steroid (cholesterol). Other tissues were pretreated with inhibitors of adenylate cyclase (SQ 22536), phosphodiesterase (rolipram), nitric oxide (NO) synthases (L-NAME) or a nuclear P4 receptor antagonist (mifepristone, MIF), followed by P4. Data were analyzed by ANOVA. Results: P4 (P 0.05) inhibits uterine contractions. R5020 and cholesterol have little P 0.05) inhibitory effects. P4 inhibition is not blocked by MIF, SQ, ODQ, rolipram or L-NAME (P 0.05). Conclusions: P4 rapidly inhibits myometrial contractility by nongenomic mechanisms through action on mPR but not via cAMP, cGMP, or NO

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Cyclic adenosine monophosphate , myometrium , preterm labor , progesterone receptors , uterine contractility

Cukurova Medical Journal

102