مرکز منطقه ای اطلاع رساني علوم و فناوري - The Levels of Oxidative Stress Biomarkers of Liver and Mammary Tissues of Apparently Healthy Mice

Abstract :

Purpose: In the present study, the levels of antioxidant systems such as glucose-6-phosphate dehydrogenase (G6PDH), catalase (CAT), superoxide dismutase (SOD), glutathione-S-transferase (GST) and, also reduced glutathione (GSH) in the liver and breast tissues were examined in order to obtain basal data for subsequent toxicological investigations. Additionally, the level of thiobarbituric acid reactive substance (TBARS) was measured in liver and breast tissues as an index of lipid peroxidation. Materials and Methods: Sixty-six apparently healthy mice (36 males, 30 females) had normal liver and breast tissues (histopathological data) were taken into the study. The tissues were homogenised with ice-cold 1.15% KCI. The activities of antioxidant enzymes, the levels of GSH and TBARS were measured as spectrophotometric in the supernatant obtained from centrifugation at 14.000 rpm. Results: The levels of antioxidant systems and TBARS in the liver were significantly higher than those found in the breast tissue of female and male mice. However, the levels of CAT, SOD, GST, GSH and TBARS in breast tissue except G6PDH enzyme were not significantly different between female and male mice. On the other hand, the levels of CAT, SOD, GST, GSH and TBARS in the male liver were higher than the female liver except for G6PDH enzyme. Conclusion: Results suggest that antioxidant defense of the liver tissue was higher compared to breast tissue possibly due to a compensatory response to various toxic substances in the liver and thereby protects the cells against oxidative damage.