Budiningrum, Anggun Indah Brawijaya University - Faculty of Mathematics and Natural Sciences - Departement of Biology, Indonesia , Rofi’i, Achmad University of Lambung Mangkurat - Faculty of Medicine, Ulin General Hospital - Departments of Otorhinolaryngology, Head and Neck, Indonesia , Suharjono, Suharjono Brawijaya University - Faculty of Mathematics and Natural Sciences - Departement of Biology, Indonesia , Fatchiyah, Fatchiyah Brawijaya University - Faculty of Mathematics and Natural Sciences - Departement of Biology, Indonesia

LMP-1 and Nasopharyngeal Carcinoma (NPC)

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Purpose: This study aims to determine the interaction of LMP-1 with H2O2 , nitrosamine, and nicotine to predict the external factor that affected LMP-1, which activates lytic phase on nasopharyngeal carcinoma (NPC).Materials and Methods: The amino acid sequence of human LMP1 (GI: 343177335) and compound structure of H_2O_2 (ID:784), nicotine (ID: 89594), and nitrosamine (ID: 37183) was obtained from database The National center of Biotechnology Information. The 3-dimension modeling structure of LMP-1 predicted using SWISS-MODEL web server. Hex 6.12 was used for the purpose of docking of the lead with the target molecule. Ligand details interaction then detected by LigPlus+ v.1.4.4 software. Molecular graphics and analysis were performed with PyMOL.Results: LMP-1 has interaction with H2O2 , nitrosamine, and nicotine. Total energy that use of LMP-1 to interaction with H2O2 (-67,79 kJ/mol) is lowest than interaction with nicotine (-149,43 kJ/mol) and nitrosamine (-82,93 kJ/mol). The kind of interaction between LMP-1 and H_2O_2 is hydrogen bond whereas interaction of LMP-1 between nicotine and nitrosamine are hydrophobic bond.Conclusions:LMP-1 has strongest interaction with H2O2 than nicotine and nitrosamine. This is predicted that H_2O_2 is external factor that affect LMP-1 switching to lytic infection in NPC patients.

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H_2O_2 , LMP , 1 , nasopharyngeal carcinoma, nicotine, nitrosamine

Cukurova Medical Journal

487